Subunits of glutamate receptors, their preparation and their use

ABSTRACT

The invention relates to novel subunits for glutamate receptors and to the DNA sequences coding therefor, and to processes for preparing DNA sequences and receptors. The invention furthermore relates to methods for identifying functional ligands for these receptors.

This application is a 371 of PCT/EP95/00290 filed 27 Jan. 95, published as WO95/21188 Aug. 10, 1995. The invention relates to the expression of novel variants of ionotropic glutamate receptor subunits in eukaryotic cells and to methods for finding functional ligands for corresponding glutamate receptor channels.

Glutamate is the most important excitatory neurotransmitter in the central nervous system (TIPS 11, 1990, 126-132; Pharmacological Reviews 40, 1989, 143-210; TIPS 13, 1992, 291-296) and is involved in numerous pathophysiological processes such as epilepsy, schizophrenia, ischemia. Glutamate receptors are therefore potential sites of attack for appropriate drugs.

To date, the primary structure has been elucidated for some subunits of AMPA, kainate and NMDA receptors and some metabotropic receptors (Nature 342, 1989, 643; Science 249, 1990, 556; Neuron 8, 1992, 169; Science 256, 1992, 1217; Nature 358, 1992, 36).

Four AMPA-glutamate receptor subunits of the rat have hitherto been described in the literature, GluRA, GluRB, GluRC and GluRD, each of which occurs in two splicing variants "flip" and "flop" (Science 249, 1990, 1580). In addition, RNA editing which affects the Q/R site of the second transmembrane domain has been shown for mouse and rat GluRB. These two GluRB variants differ considerably in their electrophysiological properties (Cell 67, 1991, 11-19; Neuron 8; 1992, 189-198). The human cDNA for GluRAflip and GluRAflop has likewise been published (PNAS U.S.A. 88, 1991, 7557-7561; PNAS USA 89, 1992, 1443-1447).

We have now found variants of the human glutamate receptor subunits A, B, C and D, as well as DNA sequences which code for such subunits. These subunits lead to GluR channels with specific electrophysiological properties.

We have found that the first amino acid of the flip/flop region of GluRA, GluRB, GluRC and GluRD can be in the form of glycine (G) or arginine (R) due to RNA editing. The names of the corresponding subunits are as follows:

GluRAflipG, GluRAflipR, GluRAflopG, GluRAflopR

GluRBflipQ-G, GluRBflipQ-R, GluRBflopQ-G, GluRBflopQ-R

GluRBflipR-G, GluRBflipR-R, GluRBflopR-G, GluRBflopR-R

GluRCflipG, GluRCflipR, GluRCflopG, GluRCflopR

GluRDflipG, GluRDflipR, GluRDflopG, GluRDflopR

In the case of GluRB the RNA editing known for the rat has been taken into account in the naming of the corresponding variants.

Furthermore, a variant of GluRA produced by alternative splicing, in which a 240 bp fragment is missing in the 5' region of the GluRA cDNA and thus the corresponding protein is truncated by 80 amino acids, has been found. The names of the corresponding subunits are as follows:

GluRAdel240flipG, GluRAdel240flipR, GluRAdel240flopG, GluRAdel240flopR

The following DNA and amino-acid sequences all relate to human glutamate receptor subunits.

SEQ ID NO: 1 depicts the cDNA sequence of GluRAflipG and the polypeptide sequence (SEQ ID NO: 2) derived therefrom;

SEQ ID NO: 3 depicts the cDNA sequence of GluRAflopG, and

SEQ ID NO: 4 depicts the polypeptide sequence derived therefrom.

Compared with the GluRAflipG cDNA, the GluRAflipR cDNA has a base exchange at position bp 2269 which converts a glycine codon (GGA) into an arginine codon (AGA). A corresponding statement applies to GluRAflopR.

The GluRAdel240 variants correspond to the said GluRA variants but have a deletion: bp 221-460 relative to SEQ ID NO: 1 and 3.

SEQ ID NO: 5 depicts the cDNA sequence of GluRBflipQ-G and the polypeptide sequence derived therefrom (SEQ ID NO: 6); SEQ ID NO: 7 depicts the cDNA sequence of GluRBflopQ-G, and SEQ ID NO: 8 depicts the polypeptide sequence derived therefrom.

Compared with GluRBflipQ-G, the cDNA for GluRBflipQ-R has a base exchange at position bp 2290 which converts a glycine codon (GGA) into an arginine codon (AGA). A corresponding statement applies to GluRBflopQ-R.

Compared with the abovementioned GluRB variants, the cDNA molecules for GluRBflipR-G, GluRBflipR-R, GluRBflopR-G and GluRBflopR-R have a base exchange at position bp 1820 which converts a glutamine codon (CAG) into an arginine codon (CGG).

SEQ ID NO: 9 depicts the cDNA sequence of GluRCflipG and

SEQ ID NO: 10 depicts the polypeptide sequence derived therefrom.

SEQ ID NO: 11 shows the cDNA sequence of GluRCflopG and

SEQ ID NO: 12 depicts the polypeptide sequence derived therefrom.

Compared with GluRCflipG and GluRCflopG, respectively, the cDNA molecules for GluRCflipR and GluRCflopR have a base exchange at position bp 2377 which converts a glycine codon (GGA) into an arginine codon (AGA).

SEQ ID NO: 13 depicts the cDNA sequence of GluRDflipG and

SEQ ID NO: 14 depicts the polypeptide sequence derived therefrom.

SEQ ID NO: 15 shows the cDNA sequence of GluRDflopG and

SEQ ID NO: 16 depicts the polypeptide sequence derived therefrom.

Compared with GluRDflipG and GluRDflopG, respectively, the cDNA molecules for GluRDflipR and GluRDflopR have a base exchange at position bp 2293 which converts a glycine codon (GGA) into an arginine codon (AGA).

Other suitable DNA sequences are those which although they have a different nucleotide sequence from that detailed in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15 code for the polypeptide chain detailed in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 or 16, or parts thereof as a consequence of the degeneracy of the genetic code. Also suitable are those DNA sequences which code for AMPA-glutamate receptor subunits and which hybridize under standard conditions with the nucleotide sequence depicted in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15 or with a nucleotide sequence which codes for the protein depicted in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 or 16. Standard conditions mean, for example, temperatures of from 42° to 58° C. in an aqueous buffer solution with a concentration of from 0.1 to 1×SSC (1×SSC: 0.15M NaCl, 15 mM sodium citrate pH 7.2). The experimental conditions for DNA hybridization are described in textbooks of genetic manipulation, for example in Sambrook et al., Molecular Cloning, Cold Spring Harbor Laboratory, 1989.

We have furthermore found genetic engineering processes for preparing these subunits. We have additionally found that the DNA sequences coding for these receptor subunits can be used to find functional ligands for these receptors. The invention furthermore relates to methods for identifying functional ligands for AMPA-glutamate receptors, which comprise transfecting cells with sequences which code for AMPA-GluR subunits, isolating the membranes of these cells, and carrying out conventional receptor-binding experiments with these membranes.

Another method according to the invention for identifying functional ligands for AMPA-glutamate receptors comprises causing in cells which have been transfected with one or more DNA sequences which code for AMPA-GluR subunits an effect on the signal transduction pathway due to binding of the ligands to the receptor, which is detected by a receptor system, for example the intracellular Ca⁺⁺ concentration after ligand binding by fluorimetric methods (Anal. Biochem. 209, 1993, 343).

The novel polypeptides and DNA sequences can be prepared by using conventional methods of genetic engineering. Thus, mRNA can be isolated from brain tissue and converted into double-stranded cDNA. This cDNA can be used as template for the polymerase chain reaction. It is thus possible by using specific primers under suitable reaction conditions to amplify the appropriate cDNA. The use of suitable primers makes it possible to sequence the amplified cDNA without previous cloning. The double-stranded cDNA can also be integrated in λ vectors, eg. λ gt 10 or λ ZAP, in order to generate a brain-specific cDNA bank. A cDNA bank of this type can be screened with radiolabeled DNA or RNA probes in order to identify clones which display homology with the hybridization probe. The methods used for this are described, for example, in Current Protocols in Molecular Biology (edited by F. M. Ausubel et al.) 1989, ISBN 0-471 50338-x (Vol. 1 and 2), for the polymerase chain reaction in Saiki et al., Science, 230 (1985) 1350-54 and Mullis and Faloona, Meth. Enzymol., 155 (1987) 335-350.

The cDNA characterized in this way can easily be obtained using restriction enzymes. The fragments resulting from this can be used, where appropriate in conjunction with chemically synthesized oligonucleotides, adaptors or gene fragments, to clone the sequences coding for the protein. Incorporation of the gene fragments or synthetic DNA sequences into cloning vectors, e.g. the commercial plasmids M13mp18 or Bluescript, is carried out in a conventional way. The genes or gene fragments can also be provided with suitable control regions which have been chemically synthesized or isolated from bacteria, phages, eukaryotic cells or their viruses and which make it possible to express the proteins in various host systems.

The transformation or transfection of suitable host organisms with hybrid plasmids has likewise been described in detail (M. Wigler et al., Cell, 16 (1979), 777-785; F. L. Graham and A. J. van der Eb, Virology, 52 (1973), 456-467).

On expression in mammalian cells it is possible to use vectors which place the gene to be expressed, in this case the cDNA sequences coding for the AMPA-glutamate receptor subunits described herein, under the control of the mouse metallothionein, the viral SV40 or the cytomegalovirus promoter (J. Page Martin, Gene, 37 (1985), 139-144). Needed for expression is the presence of the methionine start codon of the gene which codes for these subunits of AMPA-glutamate receptors. Clones which have copies of these vectors as episomes or integrated into the genome are then isolated. It is particularly advantageous to integrate the foreign gene into a vector which contains the cytomegalovirus promoter.

As an alternative to this, cells can be transfected with a suitable vector in such a way that the transient expression of the DNA introduced in this way is sufficient for pharmacological characterization of the expressed heterologous polypeptides. In this case too, control of expression by the cytomegalovirus promoter is particularly advantageous.

It is furthermore possible to prepare functional AMPA-glutamate receptors by transfecting one or more different DNA sequences from the group of AMPA-GluR subunits together into cells. AMPA-glutamate receptors with different subunits can be obtained in this way.

The use of shuttle vectors is very suitable in conjunction with prokaryotic sequences which code for replication in bacterial cells and antibiotic resistance. The construction and replication of the plasmid take place initially in bacterial cells; this is followed by transfer into eukaryotic cells, e.g. into the human embryonic kidney cell line HEK 293.

It is also possible to use other cell systems, e.g. yeast and other fungi, insect cells as well as animal and human cells such as CHO, COS and L cells in conjunction with suitable expression vectors for the expression of the cloned cDNA.

The eukaryotic expression systems have the advantage that they are able to express their products efficiently and usually in native form. They have furthermore the ability to carry out post-translational modification of their products.

The expressed receptor proteins can be solubilized by detergents and purified by affinity chromatography by conventional methods. The pure polypeptide can, after crystallization and X-ray structural analysis or other physical methods such as NMR or scanning tunneling microscopy, be used to elucidate first the spatial structure of the receptor and then the spatial structure of the ligand binding site.

The expressed receptor proteins can, after appropriate purification, also be used as antigens for generating polyclonal or monoclonal antibodies. These antibodies in turn can be used where appropriate for diagnostic purposes. Another possible use of such antibodies is as aids to rational drug design. Thus, the receptor-specific antibodies can be employed as antigen for generating anti-idiotype antibodies. Such antibodies may represent an image of defined regions of the receptor and be used for screening for specific receptor ligands or for rational drug design.

Receptor-expressing cell lines represent an important instrument in screening for specific receptor ligands. The membranes of these cells can be used for a receptor binding assay for this purpose. Information about the mode of action (agonism/antagonism) of a receptor ligand can be obtained by providing cells, which have been transfected with a DNA sequence according to the invention, with a suitable reporter system. Suitable reporter systems are those in which a promoter which is regulated by compounds of the signal transduction pathway (second messenger) is functionally connected to a gene for a product which can easily be detected, such as luciferase. Such reporter systems are disclosed, for example, in Science 252, (1991) 1424, Proc. Natl. Acad. Sci. U.S.A. 88 (1991) 5061 or Journal of Receptor Res. 13, (1993) 79. A suitable promoter which is, for example, regulated by the intracellular Ca⁺⁺ concentration is that of the fos gene. It is also possible to detect changes in the intracellular Ca⁺⁺ concentration directly using fluorescent dyes, eg. FURA 2AM.

Furthermore, the current flowing through the cell membrane as a function of the ligand binding can be measured.

Because of the degeneracy of the genetic code, it is possible to use DNA sequences other than those described here, e.g. chemically synthesized genes with a different DNA sequence, for the expression of the described subunits of human AMPA-glutamate receptors.

The invention makes it possible to identify and characterize substances which bind to the receptor described herein and there have an agonistic or antagonistic action.

The invention furthermore relates to the use of oligonucleotides which are derived from the structure described in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15 as antisense molecules for switching off genes in a targeted manner.

The invention also makes it possible to prepare synthetic oligonucleotides with which the expression of AMPA-glutamate receptor subunits can be specifically inhibited by intracerebroventricular administration, as has been described, for example, for NMDA receptors (Nature 363, 1993, 260).

Other embodiments of the invention are described in detail in the examples.

For genetic engineering methods, reference may be made, for example, to the handbook by Sambrook et al., Molecular Cloning, Cold Spring Harbor Laboratory, 1989, or DNA cloning, Vol. I to III, IRI Press 1985 to 1987, edited by D. M. Glover.

EXAMPLE 1

Isolation of CDNA molecules which code for the human AMPA-glutamate receptor subunits GluRA and GluRAdel240.

The polymerase chain reaction (PCR) technique was used to amplify three cDNA fragments which are specific for the human AMPA-glutamate receptor subunit GluRA from two commercially available human brain cDNA libraries. To increase the specificity of amplification, the 2-stage PCR method described hereinafter was carried out: in each case 10 μl of lysate from a human temporal cortex cDNA library (titer: 1.5×10¹¹ phages/ml; vector lambda ZAP; GluRA cDNA fragment bp 1-839) or a human nucleus accumbens cDNA library (titer: 1-9×10⁹ phages/ml; vector lambda gt10; GluRA cDNA fragments 1-1421 and 1407-2721) were used as templates for the first PCR reaction with the primer oligonucleotides A and B. The volumes of the reaction mixtures were 100 μl in each case, 20 pmol of each of the various primers were employed, and the reaction buffer contained 10 mM tris-HCl pH 8.5, 50 mM KCl, 1.5 mM MgCl₂, and 0.2 mM each of DATP, dCTP, dGTP and dTTP. 20 cycles with the following temperature profile were carried out: 94° C. for 1 minute, 55° C. for 1 minute, 72° C. for 1 minute. A Perkin-Elmer type 9600 thermocycler was used.

After the PCR had been carried out, in each case 10 μl of the PCR mixtures were removed and used as templates for a second PCR with primers C and D. The reaction and buffer conditions were the same as for the first PCR, but the number of cycles was increased to 35. The amplified cDNA fragments were cloned into the vector pCRII in accordance with the manufacturer's instructions using the Invitrogen TA cloning kit.

The primer oligonucleotides for amplification of the human GluRA cDNA fragment which comprises base pairs 1-1431 had the following sequences:

Primer A: 5'-ATCTATGATTGGACCTGGGC-3' (SEQ ID NO: 17)

Primer B: 5'-ACATCTGCTCTTCCATAGACCAGC-3' (SEQ ID NO: 18)

Primer C: 5'-TGCGATAAGCTTATGCAGCACATTTTTGCCTTCTTCTGC-3' (SEQ ID NO: 19)

Primer D: 5'-ATGCCATTCCAGGCCTTCGTGTCA-3' (SEQ ID NO: 20)

The primer oligonucleotides for amplification of the human GluRA cDNA fragment which comprises base pairs 1407-2721 had the following sequences:

Primer A: 5'-GATGGAAAATACGGAGCCCGA-3' (SEQ ID NO: 21)

Primer B: 5'-GCTGGGGAGCCGAGCCTGCTC-3' (SEQ ID NO: 22)

Primer C: 5'-TGACACGAAGGCCTGGAATGGCAT-3' (SEQ ID NO: 23)

Primer D: 5'-TGCGATGAATTCTTACAATCCCGTGGCTCCCAAGGGCAT-3' (SEQ ID NO: 24)

The primer oligonucleotides for amplification of the human GluRA cDNA fragment which comprises base pairs 1-839 had the following sequences:

Primer A: SEQ ID NO: 17

Primer B: 5'-TACTTGGGTCTCTTCCAGTCCA-3' (SEQ ID NO: 25)

Primer C: SEQ ID NO: 20

Primer D: 5'-TGTGTGGTCTCGAGCATCACTATT-3' (SEQ ID NO: 26)

Standard methods of genetic engineering (see, for example, Sambrook et al. (1989), Molecular Cloning, Cold Spring Harbor Laboratory) were used to assemble the amplified cDNA fragments in each case to the complete coding regions of GluRA and GluRAdel240.

EXAMPLE 2

Isolation of cDNA molecules for human glutamate receptor subunits A, B, C and D

cDNA fragments which are specific for human glutamate receptor subunits A, B, C and D were obtained by screening the following commercially available human brain cDNA libraries:

Hippocampus (from Stratagene)

Cerebellum (from Clontech and Stratagene)

Nucleus accumbens (Clontech)

The screening probes used were PCR fragments 600-3000 bp in size which had been amplified from the cDNA molecules for rat GluRA, B, C and D which had been cloned in pBluescript. In each case 1 ng of plasmid DNA was employed as template. The primer concentrations and buffer conditions for the PCRs corresponded to those in Example 1 but in each case 2 μl of the dNTP labeling mixture from the Boehringer Mannheim DNA labeling and detection kit were employed as nucleotide source. 35 cycles with the following temperature profile were carried out: 94° C. for 2 minutes, 55° C. for 2 minutes, 72° C. for 3 minutes. The Dig-dUTP-labeled fragments were purified on a Seaplaque agarose gel. The screening procedure was carried out in accordance with the instructions in the manual for the abovementioned kit. The GluR cDNA fragments of the lambda clones derived from the screening were cloned by conventional methods of genetic engineering into the vector pBluescript and assembled to give the complete cDNA molecules of the various GluR variants.

EXAMPLE 3

Transient expression of the cloned human GluR genes in HEK293 cells.

Unless stated otherwise, the cell culture was carried out as described by Lindl and Bauer, Zell-und Gewebekultur, Gustav Fischer Verlag.

The GluR cDNA molecules from Examples 1 and 2 were cloned into conventional plasmids such as pBluescript (from Stratagene) and PCRII (from Invitrogen) during their isolation. The cloned GluR fragments in each case comprise the entire open reading frame including start and stop codons and at least 40 bp of the 5' non-translated region preceding the start codon.

For the transient expression in eukaryotic cell lines, the cloned GluR fragments were cloned into the expression vector pcDNA3 (from Invitrogen). The recombinant plasmids resulting therefrom were replicated in a known manner.

HEK 293 cells were cultivated under standard conditions. After trypsinization, the cells were taken up in DMEM (Gibco) which contained 3.7 g/l NaHCO₃, and 10 cm Petri dishes were inoculated with 1.5×10⁶ cells. These cells were then cultivated at 37° C. and 5% CO₂ for 24 h.

The DNA to be transfected was prepared as follows: 20 μg of the DNA solution (1 mg/ml), purified using the Quiagen® system from Diagen, were mixed with 437 μl of H₂ O, and then 62.5 μl of 2M CaCl₂, and finally 500 μl of PBS, were added. Ca⁺⁺ precipitates formed within 10 min at room temperature.

The solution was placed on a 10 cm culture dish containing the HEK 293 cells cultivated by the above method. After cautious mixing, the cells were cultivated in an incubator at 37° C./3% CO₂ for 15 to 20 h. Then 5 ml of serum-free medium were cautiously added. After removal of all the medium and repetition of the washing process with 5 ml of medium, 10 ml of medium were added to the cells. After incubation at 37° C. and 5% CO₂ for 48 h, the cells were suitable for pharmacologial and electrophysiological investigations.

Alternatively, the DNA was also introduced into the cells with liposome mediation. Lipofectin from GIBCO-BRL was employed for this in accordance with the manufacturer's instructions.

EXAMPLE 4

Expression of the AMPA-glutamate receptor subunits in oocytes

To prepare cRNA, the corresponding cDNA molecules which code for the glutamate receptor subunits were cloned by standard protocols into the Bluescript plasmid (Stratagene) which had been cleaved with EcoRI.

Plasmid DNA was obtained by standard methods after growing of the Bluescript clones which code for subunits of AMPA-glutamate receptors. This plasmid DNA was cleaved with the restriction enzyme Not I and employed for the in vitro transcription. The transcription was started from the T3 or T7 promoter and carried out under standard conditions in accordance with the Stratagene in vitro transcription kit.

For the expression of the receptor subunits, in each case 10 ng of cRNA were injected either alone or combined with other cRNA into oocytes which had been explanted from the clawed frog Xenopus laevis C. Methfessel et al., Pflugers Arch. 407, 577, (1986)!. The oocytes were incubated in OR-2 (92.5 mM NaCl, 2.5 mM KCl, 1 mM Na₂ HPO₄, 5 mM HEPES, 1 mM MgCl₂, 1 mM CaCl₂, 0.5 g/l polyvinylpyrrolidone, pH 7.2 with the addition of 4 μg/ml Zinacef and 100 U/ml Penstrep) at 19° C. 24 hours after the injection, the oocytes were treated with collagenase (Sigma Type II) (1 mg/ml in OR-2 for 1 hour). Electrophysiological recordings were made 2-6 days after injection of the cRNA. A 2-electrode voltage clamp configuration was used for this with a TEC 01C amplifier (NPI Electronic, Tamm, Germany). During the electrophysiological measurements, the oocytes were perfused with normal frog Ringer solution (NRF: 115 mM NaCl, 2.5 mM KCl, 1.8 mM CaCl₂, 10 mM HEPES, pH 7.2).

EXAMPLE 5

Stable expression of the glutamate receptor subunits in HEK 293 cells

The glutamate receptor cDNA molecules described in Examples 1 and 2 were cloned into the eukaryotic expression vectors pcDNA3 and pRc/CMV (from Invitrogen). These expression constructs were introduced singly or in combination into HEK 293 cells by electroporation by the following protocol: HEK 293 cells (ATCC) were cultivated in RPMI 1640 medium (Glutamax I from Gibco BRL) containing 10% FCS (Gibco BRL) under 5% CO₂. For the electroporation, 10⁷ cells were transfected in 0.8 ml of PBS with 20 μg of the expression construct using an electroporator (BTX, electro cell manipulator 600, 3 μF, 130 V, 72 ohm). The cells were subsequently incubated in culture medium for 24 h and then transferred into selection medium (RPMI medium with 600 μg/ml G418 sulfate, geneticin). Stable geneticin-resistant cell clones were isolated after 10-12 days by plating out and were expanded and analyzed by a membrane binding assay.

    __________________________________________________________________________     SEQUENCE LISTING                                                               (1) GENERAL INFORMATION:                                                       (iii) NUMBER OF SEQUENCES: 26                                                  (2) INFORMATION FOR SEQ ID NO: 1:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2946 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: Adult                                                 (C) TISSUE TYPE: Brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 144..2861                                                        (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1:                                       GAAAGGAAGGAAGCAAGCAAGCAAGGAAGGAACTGCAGGAGGAAAAGAACAGGCAGAACA60                 GCGAAAAGAATAAAGGGAAAGGGGGGGAAACACCAAATCTATGATTGGACCTGGGCTTCT120                TTTTCGCCAATGCAAAAAGGAATATGCAGCACATTTTTGCCTTCTTCTGC170                          MetGlnHisIlePheAlaPhePheCys                                                    15                                                                             ACCGGTTTCCTAGGCGCGGTAGTAGGTGCCAATTTCCCCAACAATATC218                            ThrGlyPheLeuGlyAlaValValGlyAlaAsnPheProAsnAsnIle                               10152025                                                                       CAGATCGGGGGATTATTTCCAAACCAGCAGTCACAGGAACATGCTGCT266                            GlnIleGlyGlyLeuPheProAsnGlnGlnSerGlnGluHisAlaAla                               303540                                                                         TTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAAGCTGCTCCCC314                            PheArgPheAlaLeuSerGlnLeuThrGluProProLysLeuLeuPro                               455055                                                                         CAGATTGATATTGTGAACATCAGCGACAGCTTTGAGATGACCTATAGA362                            GlnIleAspIleValAsnIleSerAspSerPheGluMetThrTyrArg                               606570                                                                         TTCTGTTCCCAGTTCTCCAAAGGAGTCTATGCCATCTTTGGGTTTTAT410                            PheCysSerGlnPheSerLysGlyValTyrAlaIlePheGlyPheTyr                               758085                                                                         GAACGTAGGACTGTCAACATGCTGACCTCCTTTTGTGGGGCCCTCCAC458                            GluArgArgThrValAsnMetLeuThrSerPheCysGlyAlaLeuHis                               9095100105                                                                     GTCTGCTTCATTACGCCGAGCTTTCCCGTTGATACATCCAATCAGTTT506                            ValCysPheIleThrProSerPheProValAspThrSerAsnGlnPhe                               110115120                                                                      GTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCATCAGCATCATT554                            ValLeuGlnLeuArgProGluLeuGlnAspAlaLeuIleSerIleIle                               125130135                                                                      GACCATTACAAGTGGCAGAAATTTGTCTACATTTATGATGCCGACCGG602                            AspHisTyrLysTrpGlnLysPheValTyrIleTyrAspAlaAspArg                               140145150                                                                      GGCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCTGCTGAGAAGAAC650                            GlyLeuSerValLeuGlnLysValLeuAspThrAlaAlaGluLysAsn                               155160165                                                                      TGGCAGGTGACAGCAGTCAACATCTTGACAACCACAGAGGAGGGATAC698                            TrpGlnValThrAlaValAsnIleLeuThrThrThrGluGluGlyTyr                               170175180185                                                                   CGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTGGTG746                            ArgMetLeuPheGlnAspLeuGluLysLysLysGluArgLeuValVal                               190195200                                                                      GTGGACTGTGAATCAGAACGCCTCAATGCTATCTTGGGCCAGATTATA794                            ValAspCysGluSerGluArgLeuAsnAlaIleLeuGlyGlnIleIle                               205210215                                                                      AAGCTAGAGAAGAATGGCATCGGCTACCACTACATTCTTGCAAATCTG842                            LysLeuGluLysAsnGlyIleGlyTyrHisTyrIleLeuAlaAsnLeu                               220225230                                                                      GGCTTCATGGACATTGACTTAAACAAATTCAAGGAGAGTGGCGCCAAT890                            GlyPheMetAspIleAspLeuAsnLysPheLysGluSerGlyAlaAsn                               235240245                                                                      GTGACAGGTTTCCAGCTGGTGAACTACACAGACACTATTCCGGCCAAG938                            ValThrGlyPheGlnLeuValAsnTyrThrAspThrIleProAlaLys                               250255260265                                                                   ATCATGCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGTG986                            IleMetGlnGlnTrpLysAsnSerAspAlaArgAspHisThrArgVal                               270275280                                                                      GACTGGAAGAGACCCAAGTACACCTCTGCGCTCACCTACGATGGGGTG1034                           AspTrpLysArgProLysTyrThrSerAlaLeuThrTyrAspGlyVal                               285290295                                                                      AAGGTGATGGCTGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGAT1082                           LysValMetAlaGluAlaPheGlnSerLeuArgArgGlnArgIleAsp                               300305310                                                                      ATATCTCGCCGGGGGAATGCTGGGGATTGTCTGGCTAACCCAGCTGTT1130                           IleSerArgArgGlyAsnAlaGlyAspCysLeuAlaAsnProAlaVal                               315320325                                                                      CCCTGGGGCCAAGGGATCGACATCCAGAGAGCTCTGCAGCAGGTGCGA1178                           ProTrpGlyGlnGlyIleAspIleGlnArgAlaLeuGlnGlnValArg                               330335340345                                                                   TTTGAAGGTTTAACAGGAAACGTGCAGTTTAATGAGAAAGGACGCCGG1226                           PheGluGlyLeuThrGlyAsnValGlnPheAsnGluLysGlyArgArg                               350355360                                                                      ACCAACTACACGCTCCACGTGATTGAAATGAAACATGACAGCATCCGA1274                           ThrAsnTyrThrLeuHisValIleGluMetLysHisAspSerIleArg                               365370375                                                                      AAGATTGGTTACTGGAATGAAGATGATAAGTTTGTCCCTGCAGCCACC1322                           LysIleGlyTyrTrpAsnGluAspAspLysPheValProAlaAlaThr                               380385390                                                                      GATGCCCAAGCTGGGGGCGATAATTCAAGTGTTCAGAACAGAACATAC1370                           AspAlaGlnAlaGlyGlyAspAsnSerSerValGlnAsnArgThrTyr                               395400405                                                                      ATCGTCACAACAATCCTAGAAGATCCTTATGTGATGCTCAAGAAGAAC1418                           IleValThrThrIleLeuGluAspProTyrValMetLeuLysLysAsn                               410415420425                                                                   GCCAATCAGTTTGAGGGCAATGACCGTTACGAGGGCTACTGTGTAGAG1466                           AlaAsnGlnPheGluGlyAsnAspArgTyrGluGlyTyrCysValGlu                               430435440                                                                      CTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGAG1514                           LeuAlaAlaGluIleAlaLysHisValGlyTyrSerTyrArgLeuGlu                               445450455                                                                      ATTGTCAGTGATGGAAAATACGGAGCCCGAGACCCTGACACGAAGGCC1562                           IleValSerAspGlyLysTyrGlyAlaArgAspProAspThrLysAla                               460465470                                                                      TGGAATGGCATGGTGGGAGAGCTGGTCTATGGAAGAGCAGATGTGGCT1610                           TrpAsnGlyMetValGlyGluLeuValTyrGlyArgAlaAspValAla                               475480485                                                                      GTGGCTCCCTTAACTATCACTTTGGTCCGGGAAGAAGTTATAGATTTC1658                           ValAlaProLeuThrIleThrLeuValArgGluGluValIleAspPhe                               490495500505                                                                   TCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACCA1706                           SerLysProPheMetSerLeuGlyIleSerIleMetIleLysLysPro                               510515520                                                                      CAGAAATCCAAGCCGGGTGTCTTCTCCTTCCTTGATCCTTTGGCTTAT1754                           GlnLysSerLysProGlyValPheSerPheLeuAspProLeuAlaTyr                               525530535                                                                      GAGATTTGGATGTGCATTGTTTTTGCCTACATTGGAGTGAGTGTTGTC1802                           GluIleTrpMetCysIleValPheAlaTyrIleGlyValSerValVal                               540545550                                                                      CTCTTCCTGGTCAGCCGCTTCAGTCCCTATGAATGGCACAGTGAAGAG1850                           LeuPheLeuValSerArgPheSerProTyrGluTrpHisSerGluGlu                               555560565                                                                      TTTGAGGAAGGACGGGACCAGACAACCAGTGACCAGTCCAATGAGTTT1898                           PheGluGluGlyArgAspGlnThrThrSerAspGlnSerAsnGluPhe                               570575580585                                                                   GGGATATTCAACAGTTTGTGGTTCTCCCTGGGAGCCTTCATGCAGCAA1946                           GlyIlePheAsnSerLeuTrpPheSerLeuGlyAlaPheMetGlnGln                               590595600                                                                      GGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGTGGC1994                           GlyCysAspIleSerProArgSerLeuSerGlyArgIleValGlyGly                               605610615                                                                      GTCTGGTGGTTCTTCACCTTAATCATCATCTCCTCATATACAGCCAAT2042                           ValTrpTrpPhePheThrLeuIleIleIleSerSerTyrThrAlaAsn                               620625630                                                                      CTGGCCGCCTTCCTGACCGTGGAGAGGATGGTGTCTCCCATTGAGAGT2090                           LeuAlaAlaPheLeuThrValGluArgMetValSerProIleGluSer                               635640645                                                                      GCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTACGGGACGCTGGAA2138                           AlaGluAspLeuAlaLysGlnThrGluIleAlaTyrGlyThrLeuGlu                               650655660665                                                                   GCAGGATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTGCTGTGTTT2186                           AlaGlySerThrLysGluPhePheArgArgSerLysIleAlaValPhe                               670675680                                                                      GAGAAGATGTGGACATACATGAAGTCAGCAGAGCCATCAGTTTTTGTG2234                           GluLysMetTrpThrTyrMetLysSerAlaGluProSerValPheVal                               685690695                                                                      CGGACCACAGAGGAGGGGATGATTCGAGTGAGGAAATCCAAAGGCAAA2282                           ArgThrThrGluGluGlyMetIleArgValArgLysSerLysGlyLys                               700705710                                                                      TATGCCTACCTCCTGGAGTCCACCATGAATGAGTACATTGAGCAGCGG2330                           TyrAlaTyrLeuLeuGluSerThrMetAsnGluTyrIleGluGlnArg                               715720725                                                                      AAACCCTGTGACACCATGAAGGTGGGAGGTAACTTGGATTCCAAAGGC2378                           LysProCysAspThrMetLysValGlyGlyAsnLeuAspSerLysGly                               730735740745                                                                   TATGGCATTGCAACACCCAAGGGGTCTGCCCTGGGAGGTCCCGTAAAC2426                           TyrGlyIleAlaThrProLysGlySerAlaLeuGlyGlyProValAsn                               750755760                                                                      CTAGCGGTTTTGAAACTCAGTGAGCAAGGCGTCTTAGACAAGCTGAAA2474                           LeuAlaValLeuLysLeuSerGluGlnGlyValLeuAspLysLeuLys                               765770775                                                                      AGCAAATGGTGGTACGATAAAGGGGAATGTGGAAGCAAGGACTCCGGA2522                           SerLysTrpTrpTyrAspLysGlyGluCysGlySerLysAspSerGly                               780785790                                                                      AGTAAGGACAAGACAAGCGCTCTGAGCCTCAGCAATGTGGCAGGCGTG2570                           SerLysAspLysThrSerAlaLeuSerLeuSerAsnValAlaGlyVal                               795800805                                                                      TTCTACATCCTGATCGGAGGACTTGGACTAGCCATGCTGGTTGCCTTA2618                           PheTyrIleLeuIleGlyGlyLeuGlyLeuAlaMetLeuValAlaLeu                               810815820825                                                                   ATCGAGTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGGT2666                           IleGluPheCysTyrLysSerArgSerGluSerLysArgMetLysGly                               830835840                                                                      TTTTGTTTGATCCCACAGCAATCCATCAACGAAGCCATACGGACATCG2714                           PheCysLeuIleProGlnGlnSerIleAsnGluAlaIleArgThrSer                               845850855                                                                      ACCCTCCCCCGCAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGA2762                           ThrLeuProArgAsnSerGlyAlaGlyAlaSerSerGlyGlySerGly                               860865870                                                                      GAGAATGGTCGGGTGGTCAGCCATGACTTCCCCAAGTCCATGCAATCG2810                           GluAsnGlyArgValValSerHisAspPheProLysSerMetGlnSer                               875880885                                                                      ATTCCTTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACGGGA2858                           IleProCysMetSerHisSerSerGlyMetProLeuGlyAlaThrGly                               890895900905                                                                   TTGTAACTGGAGCAGATGGAGACCCCTTGGGGAGCAGGCTCGGCTCCCCAGCC2911                      Leu                                                                            CCATCCCAAACCCTTCAGTGCCAAAAACAACAAAA2946                                        (2) INFORMATION FOR SEQ ID NO:2:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 906 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2:                                       MetGlnHisIlePheAlaPhePheCysThrGlyPheLeuGlyAlaVal                               151015                                                                         ValGlyAlaAsnPheProAsnAsnIleGlnIleGlyGlyLeuPhePro                               202530                                                                         AsnGlnGlnSerGlnGluHisAlaAlaPheArgPheAlaLeuSerGln                               354045                                                                         LeuThrGluProProLysLeuLeuProGlnIleAspIleValAsnIle                               505560                                                                         SerAspSerPheGluMetThrTyrArgPheCysSerGlnPheSerLys                               65707580                                                                       GlyValTyrAlaIlePheGlyPheTyrGluArgArgThrValAsnMet                               859095                                                                         LeuThrSerPheCysGlyAlaLeuHisValCysPheIleThrProSer                               100105110                                                                      PheProValAspThrSerAsnGlnPheValLeuGlnLeuArgProGlu                               115120125                                                                      LeuGlnAspAlaLeuIleSerIleIleAspHisTyrLysTrpGlnLys                               130135140                                                                      PheValTyrIleTyrAspAlaAspArgGlyLeuSerValLeuGlnLys                               145150155160                                                                   ValLeuAspThrAlaAlaGluLysAsnTrpGlnValThrAlaValAsn                               165170175                                                                      IleLeuThrThrThrGluGluGlyTyrArgMetLeuPheGlnAspLeu                               180185190                                                                      GluLysLysLysGluArgLeuValValValAspCysGluSerGluArg                               195200205                                                                      LeuAsnAlaIleLeuGlyGlnIleIleLysLeuGluLysAsnGlyIle                               210215220                                                                      GlyTyrHisTyrIleLeuAlaAsnLeuGlyPheMetAspIleAspLeu                               225230235240                                                                   AsnLysPheLysGluSerGlyAlaAsnValThrGlyPheGlnLeuVal                               245250255                                                                      AsnTyrThrAspThrIleProAlaLysIleMetGlnGlnTrpLysAsn                               260265270                                                                      SerAspAlaArgAspHisThrArgValAspTrpLysArgProLysTyr                               275280285                                                                      ThrSerAlaLeuThrTyrAspGlyValLysValMetAlaGluAlaPhe                               290295300                                                                      GlnSerLeuArgArgGlnArgIleAspIleSerArgArgGlyAsnAla                               305310315320                                                                   GlyAspCysLeuAlaAsnProAlaValProTrpGlyGlnGlyIleAsp                               325330335                                                                      IleGlnArgAlaLeuGlnGlnValArgPheGluGlyLeuThrGlyAsn                               340345350                                                                      ValGlnPheAsnGluLysGlyArgArgThrAsnTyrThrLeuHisVal                               355360365                                                                      IleGluMetLysHisAspSerIleArgLysIleGlyTyrTrpAsnGlu                               370375380                                                                      AspAspLysPheValProAlaAlaThrAspAlaGlnAlaGlyGlyAsp                               385390395400                                                                   AsnSerSerValGlnAsnArgThrTyrIleValThrThrIleLeuGlu                               405410415                                                                      AspProTyrValMetLeuLysLysAsnAlaAsnGlnPheGluGlyAsn                               420425430                                                                      AspArgTyrGluGlyTyrCysValGluLeuAlaAlaGluIleAlaLys                               435440445                                                                      HisValGlyTyrSerTyrArgLeuGluIleValSerAspGlyLysTyr                               450455460                                                                      GlyAlaArgAspProAspThrLysAlaTrpAsnGlyMetValGlyGlu                               465470475480                                                                   LeuValTyrGlyArgAlaAspValAlaValAlaProLeuThrIleThr                               485490495                                                                      LeuValArgGluGluValIleAspPheSerLysProPheMetSerLeu                               500505510                                                                      GlyIleSerIleMetIleLysLysProGlnLysSerLysProGlyVal                               515520525                                                                      PheSerPheLeuAspProLeuAlaTyrGluIleTrpMetCysIleVal                               530535540                                                                      PheAlaTyrIleGlyValSerValValLeuPheLeuValSerArgPhe                               545550555560                                                                   SerProTyrGluTrpHisSerGluGluPheGluGluGlyArgAspGln                               565570575                                                                      ThrThrSerAspGlnSerAsnGluPheGlyIlePheAsnSerLeuTrp                               580585590                                                                      PheSerLeuGlyAlaPheMetGlnGlnGlyCysAspIleSerProArg                               595600605                                                                      SerLeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeu                               610615620                                                                      IleIleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrVal                               625630635640                                                                   GluArgMetValSerProIleGluSerAlaGluAspLeuAlaLysGln                               645650655                                                                      ThrGluIleAlaTyrGlyThrLeuGluAlaGlySerThrLysGluPhe                               660665670                                                                      PheArgArgSerLysIleAlaValPheGluLysMetTrpThrTyrMet                               675680685                                                                      LysSerAlaGluProSerValPheValArgThrThrGluGluGlyMet                               690695700                                                                      IleArgValArgLysSerLysGlyLysTyrAlaTyrLeuLeuGluSer                               705710715720                                                                   ThrMetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLys                               725730735                                                                      ValGlyGlyAsnLeuAspSerLysGlyTyrGlyIleAlaThrProLys                               740745750                                                                      GlySerAlaLeuGlyGlyProValAsnLeuAlaValLeuLysLeuSer                               755760765                                                                      GluGlnGlyValLeuAspLysLeuLysSerLysTrpTrpTyrAspLys                               770775780                                                                      GlyGluCysGlySerLysAspSerGlySerLysAspLysThrSerAla                               785790795800                                                                   LeuSerLeuSerAsnValAlaGlyValPheTyrIleLeuIleGlyGly                               805810815                                                                      LeuGlyLeuAlaMetLeuValAlaLeuIleGluPheCysTyrLysSer                               820825830                                                                      ArgSerGluSerLysArgMetLysGlyPheCysLeuIleProGlnGln                               835840845                                                                      SerIleAsnGluAlaIleArgThrSerThrLeuProArgAsnSerGly                               850855860                                                                      AlaGlyAlaSerSerGlyGlySerGlyGluAsnGlyArgValValSer                               865870875880                                                                   HisAspPheProLysSerMetGlnSerIleProCysMetSerHisSer                               885890895                                                                      SerGlyMetProLeuGlyAlaThrGlyLeu                                                 900905                                                                         (2) INFORMATION FOR SEQ ID NO: 3:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2946 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: Adult                                                 (C) TISSUE TYPE: Brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 144..2861                                                        (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3:                                       GAAAGGAAGGAAGCAAGCAAGCAAGGAAGGAACTGCAGGAGGAAAAGAACAGGCAGAACA60                 GCGAAAAGAATAAAGGGAAAGGGGGGGAAACACCAAATCTATGATTGGACCTGGGCTTCT120                TTTTCGCCAATGCAAAAAGGAATATGCAGCACATTTTTGCCTTCTTCTGC170                          MetGlnHisIlePheAlaPhePheCys                                                    15                                                                             ACCGGTTTCCTAGGCGCGGTAGTAGGTGCCAATTTCCCCAACAATATC218                            ThrGlyPheLeuGlyAlaValValGlyAlaAsnPheProAsnAsnIle                               10152025                                                                       CAGATCGGGGGATTATTTCCAAACCAGCAGTCACAGGAACATGCTGCT266                            GlnIleGlyGlyLeuPheProAsnGlnGlnSerGlnGluHisAlaAla                               303540                                                                         TTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAAGCTGCTCCCC314                            PheArgPheAlaLeuSerGlnLeuThrGluProProLysLeuLeuPro                               455055                                                                         CAGATTGATATTGTGAACATCAGCGACAGCTTTGAGATGACCTATAGA362                            GlnIleAspIleValAsnIleSerAspSerPheGluMetThrTyrArg                               606570                                                                         TTCTGTTCCCAGTTCTCCAAAGGAGTCTATGCCATCTTTGGGTTTTAT410                            PheCysSerGlnPheSerLysGlyValTyrAlaIlePheGlyPheTyr                               758085                                                                         GAACGTAGGACTGTCAACATGCTGACCTCCTTTTGTGGGGCCCTCCAC458                            GluArgArgThrValAsnMetLeuThrSerPheCysGlyAlaLeuHis                               9095100105                                                                     GTCTGCTTCATTACGCCGAGCTTTCCCGTTGATACATCCAATCAGTTT506                            ValCysPheIleThrProSerPheProValAspThrSerAsnGlnPhe                               110115120                                                                      GTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCATCAGCATCATT554                            ValLeuGlnLeuArgProGluLeuGlnAspAlaLeuIleSerIleIle                               125130135                                                                      GACCATTACAAGTGGCAGAAATTTGTCTACATTTATGATGCCGACCGG602                            AspHisTyrLysTrpGlnLysPheValTyrIleTyrAspAlaAspArg                               140145150                                                                      GGCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCTGCTGAGAAGAAC650                            GlyLeuSerValLeuGlnLysValLeuAspThrAlaAlaGluLysAsn                               155160165                                                                      TGGCAGGTGACAGCAGTCAACATCTTGACAACCACAGAGGAGGGATAC698                            TrpGlnValThrAlaValAsnIleLeuThrThrThrGluGluGlyTyr                               170175180185                                                                   CGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTGGTG746                            ArgMetLeuPheGlnAspLeuGluLysLysLysGluArgLeuValVal                               190195200                                                                      GTGGACTGTGAATCAGAACGCCTCAATGCTATCTTGGGCCAGATTATA794                            ValAspCysGluSerGluArgLeuAsnAlaIleLeuGlyGlnIleIle                               205210215                                                                      AAGCTAGAGAAGAATGGCATCGGCTACCACTACATTCTTGCAAATCTG842                            LysLeuGluLysAsnGlyIleGlyTyrHisTyrIleLeuAlaAsnLeu                               220225230                                                                      GGCTTCATGGACATTGACTTAAACAAATTCAAGGAGAGTGGCGCCAAT890                            GlyPheMetAspIleAspLeuAsnLysPheLysGluSerGlyAlaAsn                               235240245                                                                      GTGACAGGTTTCCAGCTGGTGAACTACACAGACACTATTCCGGCCAAG938                            ValThrGlyPheGlnLeuValAsnTyrThrAspThrIleProAlaLys                               250255260265                                                                   ATCATGCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGTG986                            IleMetGlnGlnTrpLysAsnSerAspAlaArgAspHisThrArgVal                               270275280                                                                      GACTGGAAGAGACCCAAGTACACCTCTGCGCTCACCTACGATGGGGTG1034                           AspTrpLysArgProLysTyrThrSerAlaLeuThrTyrAspGlyVal                               285290295                                                                      AAGGTGATGGCTGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGAT1082                           LysValMetAlaGluAlaPheGlnSerLeuArgArgGlnArgIleAsp                               300305310                                                                      ATATCTCGCCGGGGGAATGCTGGGGATTGTCTGGCTAACCCAGCTGTT1130                           IleSerArgArgGlyAsnAlaGlyAspCysLeuAlaAsnProAlaVal                               315320325                                                                      CCCTGGGGCCAAGGGATCGACATCCAGAGAGCTCTGCAGCAGGTGCGA1178                           ProTrpGlyGlnGlyIleAspIleGlnArgAlaLeuGlnGlnValArg                               330335340345                                                                   TTTGAAGGTTTAACAGGAAACGTGCAGTTTAATGAGAAAGGACGCCGG1226                           PheGluGlyLeuThrGlyAsnValGlnPheAsnGluLysGlyArgArg                               350355360                                                                      ACCAACTACACGCTCCACGTGATTGAAATGAAACATGACAGCATCCGA1274                           ThrAsnTyrThrLeuHisValIleGluMetLysHisAspSerIleArg                               365370375                                                                      AAGATTGGTTACTGGAATGAAGATGATAAGTTTGTCCCTGCAGCCACC1322                           LysIleGlyTyrTrpAsnGluAspAspLysPheValProAlaAlaThr                               380385390                                                                      GATGCCCAAGCTGGGGGCGATAATTCAAGTGTTCAGAACAGAACATAC1370                           AspAlaGlnAlaGlyGlyAspAsnSerSerValGlnAsnArgThrTyr                               395400405                                                                      ATCGTCACAACAATCCTAGAAGATCCTTATGTGATGCTCAAGAAGAAC1418                           IleValThrThrIleLeuGluAspProTyrValMetLeuLysLysAsn                               410415420425                                                                   GCCAATCAGTTTGAGGGCAATGACCGTTACGAGGGCTACTGTGTAGAG1466                           AlaAsnGlnPheGluGlyAsnAspArgTyrGluGlyTyrCysValGlu                               430435440                                                                      CTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGAG1514                           LeuAlaAlaGluIleAlaLysHisValGlyTyrSerTyrArgLeuGlu                               445450455                                                                      ATTGTCAGTGATGGAAAATACGGAGCCCGAGACCCTGACACGAAGGCC1562                           IleValSerAspGlyLysTyrGlyAlaArgAspProAspThrLysAla                               460465470                                                                      TGGAATGGCATGGTGGGAGAGCTGGTCTATGGAAGAGCAGATGTGGCT1610                           TrpAsnGlyMetValGlyGluLeuValTyrGlyArgAlaAspValAla                               475480485                                                                      GTGGCTCCCTTAACTATCACTTTGGTCCGGGAAGAAGTTATAGATTTC1658                           ValAlaProLeuThrIleThrLeuValArgGluGluValIleAspPhe                               490495500505                                                                   TCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACCA1706                           SerLysProPheMetSerLeuGlyIleSerIleMetIleLysLysPro                               510515520                                                                      CAGAAATCCAAGCCGGGTGTCTTCTCCTTCCTTGATCCTTTGGCTTAT1754                           GlnLysSerLysProGlyValPheSerPheLeuAspProLeuAlaTyr                               525530535                                                                      GAGATTTGGATGTGCATTGTTTTTGCCTACATTGGAGTGAGTGTTGTC1802                           GluIleTrpMetCysIleValPheAlaTyrIleGlyValSerValVal                               540545550                                                                      CTCTTCCTGGTCAGCCGCTTCAGTCCCTATGAATGGCACAGTGAAGAG1850                           LeuPheLeuValSerArgPheSerProTyrGluTrpHisSerGluGlu                               555560565                                                                      TTTGAGGAAGGACGGGACCAGACAACCAGTGACCAGTCCAATGAGTTT1898                           PheGluGluGlyArgAspGlnThrThrSerAspGlnSerAsnGluPhe                               570575580585                                                                   GGGATATTCAACAGTTTGTGGTTCTCCCTGGGAGCCTTCATGCAGCAA1946                           GlyIlePheAsnSerLeuTrpPheSerLeuGlyAlaPheMetGlnGln                               590595600                                                                      GGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGTGGC1994                           GlyCysAspIleSerProArgSerLeuSerGlyArgIleValGlyGly                               605610615                                                                      GTCTGGTGGTTCTTCACCTTAATCATCATCTCCTCATATACAGCCAAT2042                           ValTrpTrpPhePheThrLeuIleIleIleSerSerTyrThrAlaAsn                               620625630                                                                      CTGGCCGCCTTCCTGACCGTGGAGAGGATGGTGTCTCCCATTGAGAGT2090                           LeuAlaAlaPheLeuThrValGluArgMetValSerProIleGluSer                               635640645                                                                      GCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTACGGGACGCTGGAA2138                           AlaGluAspLeuAlaLysGlnThrGluIleAlaTyrGlyThrLeuGlu                               650655660665                                                                   GCAGGATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTGCTGTGTTT2186                           AlaGlySerThrLysGluPhePheArgArgSerLysIleAlaValPhe                               670675680                                                                      GAGAAGATGTGGACATACATGAAGTCAGCAGAGCCATCAGTTTTTGTG2234                           GluLysMetTrpThrTyrMetLysSerAlaGluProSerValPheVal                               685690695                                                                      CGGACCACAGAGGAGGGGATGATTCGAGTGAGGAAATCCAAAGGCAAA2282                           ArgThrThrGluGluGlyMetIleArgValArgLysSerLysGlyLys                               700705710                                                                      TATGCCTACCTCCTGGAGTCCACCATGAATGAGTACATTGAGCAGCGG2330                           TyrAlaTyrLeuLeuGluSerThrMetAsnGluTyrIleGluGlnArg                               715720725                                                                      AAACCCTGTGACACCATGAAGGTGGGAGGTAACTTGGATTCCAAAGGC2378                           LysProCysAspThrMetLysValGlyGlyAsnLeuAspSerLysGly                               730735740745                                                                   TATGGCATTGCAACACCCAAGGGGTCTGCCCTGGGAAATCCAGTAAAC2426                           TyrGlyIleAlaThrProLysGlySerAlaLeuGlyAsnProValAsn                               750755760                                                                      CTGGCAGTGTTAAAACTAAACGAGCAGGGGCTTTTGGACAAATTGAAA2474                           LeuAlaValLeuLysLeuAsnGluGlnGlyLeuLeuAspLysLeuLys                               765770775                                                                      AACAAATGGTGGTACGACAAGGGCGAGTGCGGCAGCGGGGGAGGTGAT2522                           AsnLysTrpTrpTyrAspLysGlyGluCysGlySerGlyGlyGlyAsp                               780785790                                                                      TCCAAGGACAAGACAAGCGCTCTGAGCCTCAGCAATGTGGCAGGCGTG2570                           SerLysAspLysThrSerAlaLeuSerLeuSerAsnValAlaGlyVal                               795800805                                                                      TTCTACATCCTGATCGGAGGACTTGGACTAGCCATGCTGGTTGCCTTA2618                           PheTyrIleLeuIleGlyGlyLeuGlyLeuAlaMetLeuValAlaLeu                               810815820825                                                                   ATCGAGTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGGT2666                           IleGluPheCysTyrLysSerArgSerGluSerLysArgMetLysGly                               830835840                                                                      TTTTGTTTGATCCCACAGCAATCCATCAACGAAGCCATACGGACATCG2714                           PheCysLeuIleProGlnGlnSerIleAsnGluAlaIleArgThrSer                               845850855                                                                      ACCCTCCCCCGCAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGA2762                           ThrLeuProArgAsnSerGlyAlaGlyAlaSerSerGlyGlySerGly                               860865870                                                                      GAGAATGGTCGGGTGGTCAGCCATGACTTCCCCAAGTCCATGCAATCG2810                           GluAsnGlyArgValValSerHisAspPheProLysSerMetGlnSer                               875880885                                                                      ATTCCTTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACGGGA2858                           IleProCysMetSerHisSerSerGlyMetProLeuGlyAlaThrGly                               890895900905                                                                   TTGTAACTGGAGCAGATGGAGACCCCTTGGGGAGCAGGCTCGGCTCCCCAGCC2911                      Leu                                                                            CCATCCCAAACCCTTCAGTGCCAAAAACAACAAAA2946                                        (2) INFORMATION FOR SEQ ID NO:4:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 906 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:                                       MetGlnHisIlePheAlaPhePheCysThrGlyPheLeuGlyAlaVal                               151015                                                                         ValGlyAlaAsnPheProAsnAsnIleGlnIleGlyGlyLeuPhePro                               202530                                                                         AsnGlnGlnSerGlnGluHisAlaAlaPheArgPheAlaLeuSerGln                               354045                                                                         LeuThrGluProProLysLeuLeuProGlnIleAspIleValAsnIle                               505560                                                                         SerAspSerPheGluMetThrTyrArgPheCysSerGlnPheSerLys                               65707580                                                                       GlyValTyrAlaIlePheGlyPheTyrGluArgArgThrValAsnMet                               859095                                                                         LeuThrSerPheCysGlyAlaLeuHisValCysPheIleThrProSer                               100105110                                                                      PheProValAspThrSerAsnGlnPheValLeuGlnLeuArgProGlu                               115120125                                                                      LeuGlnAspAlaLeuIleSerIleIleAspHisTyrLysTrpGlnLys                               130135140                                                                      PheValTyrIleTyrAspAlaAspArgGlyLeuSerValLeuGlnLys                               145150155160                                                                   ValLeuAspThrAlaAlaGluLysAsnTrpGlnValThrAlaValAsn                               165170175                                                                      IleLeuThrThrThrGluGluGlyTyrArgMetLeuPheGlnAspLeu                               180185190                                                                      GluLysLysLysGluArgLeuValValValAspCysGluSerGluArg                               195200205                                                                      LeuAsnAlaIleLeuGlyGlnIleIleLysLeuGluLysAsnGlyIle                               210215220                                                                      GlyTyrHisTyrIleLeuAlaAsnLeuGlyPheMetAspIleAspLeu                               225230235240                                                                   AsnLysPheLysGluSerGlyAlaAsnValThrGlyPheGlnLeuVal                               245250255                                                                      AsnTyrThrAspThrIleProAlaLysIleMetGlnGlnTrpLysAsn                               260265270                                                                      SerAspAlaArgAspHisThrArgValAspTrpLysArgProLysTyr                               275280285                                                                      ThrSerAlaLeuThrTyrAspGlyValLysValMetAlaGluAlaPhe                               290295300                                                                      GlnSerLeuArgArgGlnArgIleAspIleSerArgArgGlyAsnAla                               305310315320                                                                   GlyAspCysLeuAlaAsnProAlaValProTrpGlyGlnGlyIleAsp                               325330335                                                                      IleGlnArgAlaLeuGlnGlnValArgPheGluGlyLeuThrGlyAsn                               340345350                                                                      ValGlnPheAsnGluLysGlyArgArgThrAsnTyrThrLeuHisVal                               355360365                                                                      IleGluMetLysHisAspSerIleArgLysIleGlyTyrTrpAsnGlu                               370375380                                                                      AspAspLysPheValProAlaAlaThrAspAlaGlnAlaGlyGlyAsp                               385390395400                                                                   AsnSerSerValGlnAsnArgThrTyrIleValThrThrIleLeuGlu                               405410415                                                                      AspProTyrValMetLeuLysLysAsnAlaAsnGlnPheGluGlyAsn                               420425430                                                                      AspArgTyrGluGlyTyrCysValGluLeuAlaAlaGluIleAlaLys                               435440445                                                                      HisValGlyTyrSerTyrArgLeuGluIleValSerAspGlyLysTyr                               450455460                                                                      GlyAlaArgAspProAspThrLysAlaTrpAsnGlyMetValGlyGlu                               465470475480                                                                   LeuValTyrGlyArgAlaAspValAlaValAlaProLeuThrIleThr                               485490495                                                                      LeuValArgGluGluValIleAspPheSerLysProPheMetSerLeu                               500505510                                                                      GlyIleSerIleMetIleLysLysProGlnLysSerLysProGlyVal                               515520525                                                                      PheSerPheLeuAspProLeuAlaTyrGluIleTrpMetCysIleVal                               530535540                                                                      PheAlaTyrIleGlyValSerValValLeuPheLeuValSerArgPhe                               545550555560                                                                   SerProTyrGluTrpHisSerGluGluPheGluGluGlyArgAspGln                               565570575                                                                      ThrThrSerAspGlnSerAsnGluPheGlyIlePheAsnSerLeuTrp                               580585590                                                                      PheSerLeuGlyAlaPheMetGlnGlnGlyCysAspIleSerProArg                               595600605                                                                      SerLeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeu                               610615620                                                                      IleIleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrVal                               625630635640                                                                   GluArgMetValSerProIleGluSerAlaGluAspLeuAlaLysGln                               645650655                                                                      ThrGluIleAlaTyrGlyThrLeuGluAlaGlySerThrLysGluPhe                               660665670                                                                      PheArgArgSerLysIleAlaValPheGluLysMetTrpThrTyrMet                               675680685                                                                      LysSerAlaGluProSerValPheValArgThrThrGluGluGlyMet                               690695700                                                                      IleArgValArgLysSerLysGlyLysTyrAlaTyrLeuLeuGluSer                               705710715720                                                                   ThrMetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLys                               725730735                                                                      ValGlyGlyAsnLeuAspSerLysGlyTyrGlyIleAlaThrProLys                               740745750                                                                      GlySerAlaLeuGlyAsnProValAsnLeuAlaValLeuLysLeuAsn                               755760765                                                                      GluGlnGlyLeuLeuAspLysLeuLysAsnLysTrpTrpTyrAspLys                               770775780                                                                      GlyGluCysGlySerGlyGlyGlyAspSerLysAspLysThrSerAla                               785790795800                                                                   LeuSerLeuSerAsnValAlaGlyValPheTyrIleLeuIleGlyGly                               805810815                                                                      LeuGlyLeuAlaMetLeuValAlaLeuIleGluPheCysTyrLysSer                               820825830                                                                      ArgSerGluSerLysArgMetLysGlyPheCysLeuIleProGlnGln                               835840845                                                                      SerIleAsnGluAlaIleArgThrSerThrLeuProArgAsnSerGly                               850855860                                                                      AlaGlyAlaSerSerGlyGlySerGlyGluAsnGlyArgValValSer                               865870875880                                                                   HisAspPheProLysSerMetGlnSerIleProCysMetSerHisSer                               885890895                                                                      SerGlyMetProLeuGlyAlaThrGlyLeu                                                 900905                                                                         (2) INFORMATION FOR SEQ ID NO: 5:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2955 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: Adult                                                 (C) TISSUE TYPE: Brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 28..2676                                                         (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:                                       TTTGTCGACGCTCTACTTTTCTTGGAAATGCAAAAGATTATGCATGTTTCT51                          MetGlnLysIleMetHisValSer                                                       15                                                                             GTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTTCT99                             ValLeuLeuSerProValLeuTrpGlyLeuIlePheGlyValSerSer                               101520                                                                         AACAGCATACAGATAGGGGGGCTATTTCCTAGGGGCGCCGATCAAGAA147                            AsnSerIleGlnIleGlyGlyLeuPheProArgGlyAlaAspGlnGlu                               25303540                                                                       TACAGTGCATTTCGAGTAGGGATGGTTCAGTTTTCCACTTCGGAGTTC195                            TyrSerAlaPheArgValGlyMetValGlnPheSerThrSerGluPhe                               455055                                                                         AGACTGACACCCCACATCGACAATTTGGAGGTGGCAAACAGCTTCGCA243                            ArgLeuThrProHisIleAspAsnLeuGluValAlaAsnSerPheAla                               606570                                                                         GTCACTAATGCTTTCTGCTCCCAGTTTTCGAGAGGAGTCTATGCTATT291                            ValThrAsnAlaPheCysSerGlnPheSerArgGlyValTyrAlaIle                               758085                                                                         TTTGGATTTTATGACAAGAAGTCTGTAAATACCATCACATCATTTTGC339                            PheGlyPheTyrAspLysLysSerValAsnThrIleThrSerPheCys                               9095100                                                                        GGAACACTCCACGTCTCCTTCATCACTCCCAGCTTCCCAACAGATGGC387                            GlyThrLeuHisValSerPheIleThrProSerPheProThrAspGly                               105110115120                                                                   ACACATCCATTTGTCATTCAGATGAGACCCGACCTCAAAGGAGCTCTC435                            ThrHisProPheValIleGlnMetArgProAspLeuLysGlyAlaLeu                               125130135                                                                      CTTAGCTTGATTGAATACTATCAATGGGACAAGTTTGCATACCTCTAT483                            LeuSerLeuIleGluTyrTyrGlnTrpAspLysPheAlaTyrLeuTyr                               140145150                                                                      GACAGTGACAGAGGCTTATCAACACTGCAAGCTGTGCTGGATTCTGCT531                            AspSerAspArgGlyLeuSerThrLeuGlnAlaValLeuAspSerAla                               155160165                                                                      GCTGAAAAGAAATGGCAAGTGACTGCTATCAATGTGGGAAACATTAAC579                            AlaGluLysLysTrpGlnValThrAlaIleAsnValGlyAsnIleAsn                               170175180                                                                      AATGACAAGAAAGATGAGATGTACCGATCACTTTTTCAAGATCTGGAG627                            AsnAspLysLysAspGluMetTyrArgSerLeuPheGlnAspLeuGlu                               185190195200                                                                   TTAAAAAAGGAACGGCGTGTAATTCTGGACTGTGAAAGGGATAAAGTA675                            LeuLysLysGluArgArgValIleLeuAspCysGluArgAspLysVal                               205210215                                                                      AACGACATTGTAGACCAGGTTATTACCATTGGAAAACACGTTAAAGGG723                            AsnAspIleValAspGlnValIleThrIleGlyLysHisValLysGly                               220225230                                                                      TACCACTACATCATTGCAAATCTGGGATTTACTGATGGAGACCTATTA771                            TyrHisTyrIleIleAlaAsnLeuGlyPheThrAspGlyAspLeuLeu                               235240245                                                                      AAAATCCAGTTTGGAGGTGCAAATGTCTCTGGATTTCAGATAGTGGAC819                            LysIleGlnPheGlyGlyAlaAsnValSerGlyPheGlnIleValAsp                               250255260                                                                      TATGATGATTCGTTGGTATCTAAATTTATAGAAAGATGGTCAACACTG867                            TyrAspAspSerLeuValSerLysPheIleGluArgTrpSerThrLeu                               265270275280                                                                   GAAGAAAAAGAATACCCTGGAGCTCACACAACAACAATTAAGTATACT915                            GluGluLysGluTyrProGlyAlaHisThrThrThrIleLysTyrThr                               285290295                                                                      TCTGCTCTGACCTATGATGCCGTTCAAGTGATGACTGAAGCCTTCCGC963                            SerAlaLeuThrTyrAspAlaValGlnValMetThrGluAlaPheArg                               300305310                                                                      AACCTAAGGAAGCAAAGAATTGAAATCTCCCGAAGGGGGAATGCAGGA1011                           AsnLeuArgLysGlnArgIleGluIleSerArgArgGlyAsnAlaGly                               315320325                                                                      GACTGTCTGGCAAACCCAGCAGTGCCCTGGGGACAAGGTGTAGAAATA1059                           AspCysLeuAlaAsnProAlaValProTrpGlyGlnGlyValGluIle                               330335340                                                                      GAAAGGGCCCTCAAACAGGTTCAGGTTGAAGGTCTCTCAGGAAATATA1107                           GluArgAlaLeuLysGlnValGlnValGluGlyLeuSerGlyAsnIle                               345350355360                                                                   AAGTTTGACCAGAATGGAAAAAGAATAAACTATACAATTAACATCATG1155                           LysPheAspGlnAsnGlyLysArgIleAsnTyrThrIleAsnIleMet                               365370375                                                                      GAGCTCAAAACTAATGGGCCCCGGAAGATTGGCTACTGGAGTGAAGTG1203                           GluLeuLysThrAsnGlyProArgLysIleGlyTyrTrpSerGluVal                               380385390                                                                      GACAAAATGGTTGTTACCCTTACTGAGCTCCCTTCTGGAAATGACACC1251                           AspLysMetValValThrLeuThrGluLeuProSerGlyAsnAspThr                               395400405                                                                      TCTGGGCTTGAGAATAAGACTGTTGTTGTCACCACAATTTTGGAATCT1299                           SerGlyLeuGluAsnLysThrValValValThrThrIleLeuGluSer                               410415420                                                                      CCGTATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGCAATGAG1347                           ProTyrValMetMetLysLysAsnHisGluMetLeuGluGlyAsnGlu                               425430435440                                                                   CGCTATGAGGGCTACTGTGTTGACCTGGCTGCAGAAATCGCCAAACAT1395                           ArgTyrGluGlyTyrCysValAspLeuAlaAlaGluIleAlaLysHis                               445450455                                                                      TGTGGGTTCAAGTACAAGTTGACAATTGTTGGTGATGGCAAGTATGGG1443                           CysGlyPheLysTyrLysLeuThrIleValGlyAspGlyLysTyrGly                               460465470                                                                      GCCAGGGATGCAGACACGAAAATTTGGAATGGGATGGTTGGAGAACTT1491                           AlaArgAspAlaAspThrLysIleTrpAsnGlyMetValGlyGluLeu                               475480485                                                                      GTATATGGGAAAGCTGATATTGCAATTGCTCCATTAACTATTACCCTT1539                           ValTyrGlyLysAlaAspIleAlaIleAlaProLeuThrIleThrLeu                               490495500                                                                      GTGAGAGAAGAGGTGATTGACTTCTCAAAGCCCTTCATGAGCCTCGGG1587                           ValArgGluGluValIleAspPheSerLysProPheMetSerLeuGly                               505510515520                                                                   ATATCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTT1635                           IleSerIleMetIleLysLysProGlnLysSerLysProGlyValPhe                               525530535                                                                      TCCTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTTTTT1683                           SerPheLeuAspProLeuAlaTyrGluIleTrpMetCysIleValPhe                               540545550                                                                      GCCTACATTGGGGTCAGTGTAGTTTTATTCCTGGTCAGCAGATTTAGC1731                           AlaTyrIleGlyValSerValValLeuPheLeuValSerArgPheSer                               555560565                                                                      CCCTACGAGTGGCACACTGAGGAGTTTGAAGATGGAAGAGAAACACAA1779                           ProTyrGluTrpHisThrGluGluPheGluAspGlyArgGluThrGln                               570575580                                                                      AGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTT1827                           SerSerGluSerThrAsnGluPheGlyIlePheAsnSerLeuTrpPhe                               585590595600                                                                   TCCTTGGGTGCCTTTATGCGGCAAGGATGCGATATTTCGCCAAGATCC1875                           SerLeuGlyAlaPheMetArgGlnGlyCysAspIleSerProArgSer                               605610615                                                                      CTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACCCTGATC1923                           LeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeuIle                               620625630                                                                      ATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAG1971                           IleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrValGlu                               635640645                                                                      AGGATGGTGTCTCCCATCGAAAGTGCTGAGGATCTTTCTAAGCAAACA2019                           ArgMetValSerProIleGluSerAlaGluAspLeuSerLysGlnThr                               650655660                                                                      GAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAAAGAGTTTTTC2067                           GluIleAlaTyrGlyThrLeuAspSerGlySerThrLysGluPhePhe                               665670675680                                                                   AGGAGATCTAAAATTGCAGTGTTTGATAAAATGTGGACCTACATGCGG2115                           ArgArgSerLysIleAlaValPheAspLysMetTrpThrTyrMetArg                               685690695                                                                      AGTGCGGAGCCCTCTGTGTTTGTGAGGACTACGGCCGAAGGGGTGGCT2163                           SerAlaGluProSerValPheValArgThrThrAlaGluGlyValAla                               700705710                                                                      AGAGTGCGGAAGTCCAAAGGGAAATATGCCTACTTGTTGGAGTCCACG2211                           ArgValArgLysSerLysGlyLysTyrAlaTyrLeuLeuGluSerThr                               715720725                                                                      ATGAACGAGTACATTGAGCAAAGGAAGCCTTGCGACACCATGAAAGTT2259                           MetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLysVal                               730735740                                                                      GGTGGAAACCTGGATTCCAAAGGCTATGGCATCGCAACACCTAAAGGA2307                           GlyGlyAsnLeuAspSerLysGlyTyrGlyIleAlaThrProLysGly                               745750755760                                                                   TCCTCATTAGGAACCCCAGTAAATCTTGCAGTATTGAAACTCAGTGAG2355                           SerSerLeuGlyThrProValAsnLeuAlaValLeuLysLeuSerGlu                               765770775                                                                      CAAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGT2403                           GlnGlyValLeuAspLysLeuLysAsnLysTrpTrpTyrAspLysGly                               780785790                                                                      GAATGTGGAGCCAAGGACTCTGGAAGTAAGGAAAAGACCAGTGCCCTC2451                           GluCysGlyAlaLysAspSerGlySerLysGluLysThrSerAlaLeu                               795800805                                                                      AGTCTGAGCAACGTTGCTGGAGTATTCTACATCCTTGTCGGGGGCCTT2499                           SerLeuSerAsnValAlaGlyValPheTyrIleLeuValGlyGlyLeu                               810815820                                                                      GGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG2547                           GlyLeuAlaMetLeuValAlaLeuIleGluPheCysTyrLysSerArg                               825830835840                                                                   GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATGCACAGAATATTAAC2595                           AlaGluAlaLysArgMetLysValAlaLysAsnAlaGlnAsnIleAsn                               845850855                                                                      CCATCTTCCTCGCAGAATTCACAGAATTTTGCAACTTATAAGGAAGGT2643                           ProSerSerSerGlnAsnSerGlnAsnPheAlaThrTyrLysGluGly                               860865870                                                                      TACAACGTATATGGCATCGAAAGTGTTAAAATTTAGGGGATGACCTTGAAATG2696                      TyrAsnValTyrGlyIleGluSerValLysIle                                              875880                                                                         ATGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTACTGGAGAAAATGGACGTG2756               TTATGACTCCAGAATTTCCCAAAGCAGTGCATGCTGTCCCTTACGTGAGTCCTGGCATGG2816               GAATGAATGTCAGTGTGACTGATCTCTCGTGATTGATAAGAACCTTTTGAGTGCCTTACA2876               CAATGGTTTTCTTGTGTTTATTGTCAAAGTGGTGAGAGGCATCCAGTATCTTGAAGACTT2936               TTCTTTCAGCCAAGAATTC2955                                                        (2) INFORMATION FOR SEQ ID NO:6:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 883 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:                                       MetGlnLysIleMetHisValSerValLeuLeuSerProValLeuTrp                               151015                                                                         GlyLeuIlePheGlyValSerSerAsnSerIleGlnIleGlyGlyLeu                               202530                                                                         PheProArgGlyAlaAspGlnGluTyrSerAlaPheArgValGlyMet                               354045                                                                         ValGlnPheSerThrSerGluPheArgLeuThrProHisIleAspAsn                               505560                                                                         LeuGluValAlaAsnSerPheAlaValThrAsnAlaPheCysSerGln                               65707580                                                                       PheSerArgGlyValTyrAlaIlePheGlyPheTyrAspLysLysSer                               859095                                                                         ValAsnThrIleThrSerPheCysGlyThrLeuHisValSerPheIle                               100105110                                                                      ThrProSerPheProThrAspGlyThrHisProPheValIleGlnMet                               115120125                                                                      ArgProAspLeuLysGlyAlaLeuLeuSerLeuIleGluTyrTyrGln                               130135140                                                                      TrpAspLysPheAlaTyrLeuTyrAspSerAspArgGlyLeuSerThr                               145150155160                                                                   LeuGlnAlaValLeuAspSerAlaAlaGluLysLysTrpGlnValThr                               165170175                                                                      AlaIleAsnValGlyAsnIleAsnAsnAspLysLysAspGluMetTyr                               180185190                                                                      ArgSerLeuPheGlnAspLeuGluLeuLysLysGluArgArgValIle                               195200205                                                                      LeuAspCysGluArgAspLysValAsnAspIleValAspGlnValIle                               210215220                                                                      ThrIleGlyLysHisValLysGlyTyrHisTyrIleIleAlaAsnLeu                               225230235240                                                                   GlyPheThrAspGlyAspLeuLeuLysIleGlnPheGlyGlyAlaAsn                               245250255                                                                      ValSerGlyPheGlnIleValAspTyrAspAspSerLeuValSerLys                               260265270                                                                      PheIleGluArgTrpSerThrLeuGluGluLysGluTyrProGlyAla                               275280285                                                                      HisThrThrThrIleLysTyrThrSerAlaLeuThrTyrAspAlaVal                               290295300                                                                      GlnValMetThrGluAlaPheArgAsnLeuArgLysGlnArgIleGlu                               305310315320                                                                   IleSerArgArgGlyAsnAlaGlyAspCysLeuAlaAsnProAlaVal                               325330335                                                                      ProTrpGlyGlnGlyValGluIleGluArgAlaLeuLysGlnValGln                               340345350                                                                      ValGluGlyLeuSerGlyAsnIleLysPheAspGlnAsnGlyLysArg                               355360365                                                                      IleAsnTyrThrIleAsnIleMetGluLeuLysThrAsnGlyProArg                               370375380                                                                      LysIleGlyTyrTrpSerGluValAspLysMetValValThrLeuThr                               385390395400                                                                   GluLeuProSerGlyAsnAspThrSerGlyLeuGluAsnLysThrVal                               405410415                                                                      ValValThrThrIleLeuGluSerProTyrValMetMetLysLysAsn                               420425430                                                                      HisGluMetLeuGluGlyAsnGluArgTyrGluGlyTyrCysValAsp                               435440445                                                                      LeuAlaAlaGluIleAlaLysHisCysGlyPheLysTyrLysLeuThr                               450455460                                                                      IleValGlyAspGlyLysTyrGlyAlaArgAspAlaAspThrLysIle                               465470475480                                                                   TrpAsnGlyMetValGlyGluLeuValTyrGlyLysAlaAspIleAla                               485490495                                                                      IleAlaProLeuThrIleThrLeuValArgGluGluValIleAspPhe                               500505510                                                                      SerLysProPheMetSerLeuGlyIleSerIleMetIleLysLysPro                               515520525                                                                      GlnLysSerLysProGlyValPheSerPheLeuAspProLeuAlaTyr                               530535540                                                                      GluIleTrpMetCysIleValPheAlaTyrIleGlyValSerValVal                               545550555560                                                                   LeuPheLeuValSerArgPheSerProTyrGluTrpHisThrGluGlu                               565570575                                                                      PheGluAspGlyArgGluThrGlnSerSerGluSerThrAsnGluPhe                               580585590                                                                      GlyIlePheAsnSerLeuTrpPheSerLeuGlyAlaPheMetArgGln                               595600605                                                                      GlyCysAspIleSerProArgSerLeuSerGlyArgIleValGlyGly                               610615620                                                                      ValTrpTrpPhePheThrLeuIleIleIleSerSerTyrThrAlaAsn                               625630635640                                                                   LeuAlaAlaPheLeuThrValGluArgMetValSerProIleGluSer                               645650655                                                                      AlaGluAspLeuSerLysGlnThrGluIleAlaTyrGlyThrLeuAsp                               660665670                                                                      SerGlySerThrLysGluPhePheArgArgSerLysIleAlaValPhe                               675680685                                                                      AspLysMetTrpThrTyrMetArgSerAlaGluProSerValPheVal                               690695700                                                                      ArgThrThrAlaGluGlyValAlaArgValArgLysSerLysGlyLys                               705710715720                                                                   TyrAlaTyrLeuLeuGluSerThrMetAsnGluTyrIleGluGlnArg                               725730735                                                                      LysProCysAspThrMetLysValGlyGlyAsnLeuAspSerLysGly                               740745750                                                                      TyrGlyIleAlaThrProLysGlySerSerLeuGlyThrProValAsn                               755760765                                                                      LeuAlaValLeuLysLeuSerGluGlnGlyValLeuAspLysLeuLys                               770775780                                                                      AsnLysTrpTrpTyrAspLysGlyGluCysGlyAlaLysAspSerGly                               785790795800                                                                   SerLysGluLysThrSerAlaLeuSerLeuSerAsnValAlaGlyVal                               805810815                                                                      PheTyrIleLeuValGlyGlyLeuGlyLeuAlaMetLeuValAlaLeu                               820825830                                                                      IleGluPheCysTyrLysSerArgAlaGluAlaLysArgMetLysVal                               835840845                                                                      AlaLysAsnAlaGlnAsnIleAsnProSerSerSerGlnAsnSerGln                               850855860                                                                      AsnPheAlaThrTyrLysGluGlyTyrAsnValTyrGlyIleGluSer                               865870875880                                                                   ValLysIle                                                                      (2) INFORMATION FOR SEQ ID NO: 7:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2955 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: Adult                                                 (C) TISSUE TYPE: Brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 28..2676                                                         (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7:                                       TTTGTCGACGCTCTACTTTTCTTGGAAATGCAAAAGATTATGCATGTTTCT51                          MetGlnLysIleMetHisValSer                                                       15                                                                             GTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTTCT99                             ValLeuLeuSerProValLeuTrpGlyLeuIlePheGlyValSerSer                               101520                                                                         AACAGCATACAGATAGGGGGGCTATTTCCTAGGGGCGCCGATCAAGAA147                            AsnSerIleGlnIleGlyGlyLeuPheProArgGlyAlaAspGlnGlu                               25303540                                                                       TACAGTGCATTTCGAGTAGGGATGGTTCAGTTTTCCACTTCGGAGTTC195                            TyrSerAlaPheArgValGlyMetValGlnPheSerThrSerGluPhe                               455055                                                                         AGACTGACACCCCACATCGACAATTTGGAGGTGGCAAACAGCTTCGCA243                            ArgLeuThrProHisIleAspAsnLeuGluValAlaAsnSerPheAla                               606570                                                                         GTCACTAATGCTTTCTGCTCCCAGTTTTCGAGAGGAGTCTATGCTATT291                            ValThrAsnAlaPheCysSerGlnPheSerArgGlyValTyrAlaIle                               758085                                                                         TTTGGATTTTATGACAAGAAGTCTGTAAATACCATCACATCATTTTGC339                            PheGlyPheTyrAspLysLysSerValAsnThrIleThrSerPheCys                               9095100                                                                        GGAACACTCCACGTCTCCTTCATCACTCCCAGCTTCCCAACAGATGGC387                            GlyThrLeuHisValSerPheIleThrProSerPheProThrAspGly                               105110115120                                                                   ACACATCCATTTGTCATTCAGATGAGACCCGACCTCAAAGGAGCTCTC435                            ThrHisProPheValIleGlnMetArgProAspLeuLysGlyAlaLeu                               125130135                                                                      CTTAGCTTGATTGAATACTATCAATGGGACAAGTTTGCATACCTCTAT483                            LeuSerLeuIleGluTyrTyrGlnTrpAspLysPheAlaTyrLeuTyr                               140145150                                                                      GACAGTGACAGAGGCTTATCAACACTGCAAGCTGTGCTGGATTCTGCT531                            AspSerAspArgGlyLeuSerThrLeuGlnAlaValLeuAspSerAla                               155160165                                                                      GCTGAAAAGAAATGGCAAGTGACTGCTATCAATGTGGGAAACATTAAC579                            AlaGluLysLysTrpGlnValThrAlaIleAsnValGlyAsnIleAsn                               170175180                                                                      AATGACAAGAAAGATGAGATGTACCGATCACTTTTTCAAGATCTGGAG627                            AsnAspLysLysAspGluMetTyrArgSerLeuPheGlnAspLeuGlu                               185190195200                                                                   TTAAAAAAGGAACGGCGTGTAATTCTGGACTGTGAAAGGGATAAAGTA675                            LeuLysLysGluArgArgValIleLeuAspCysGluArgAspLysVal                               205210215                                                                      AACGACATTGTAGACCAGGTTATTACCATTGGAAAACACGTTAAAGGG723                            AsnAspIleValAspGlnValIleThrIleGlyLysHisValLysGly                               220225230                                                                      TACCACTACATCATTGCAAATCTGGGATTTACTGATGGAGACCTATTA771                            TyrHisTyrIleIleAlaAsnLeuGlyPheThrAspGlyAspLeuLeu                               235240245                                                                      AAAATCCAGTTTGGAGGTGCAAATGTCTCTGGATTTCAGATAGTGGAC819                            LysIleGlnPheGlyGlyAlaAsnValSerGlyPheGlnIleValAsp                               250255260                                                                      TATGATGATTCGTTGGTATCTAAATTTATAGAAAGATGGTCAACACTG867                            TyrAspAspSerLeuValSerLysPheIleGluArgTrpSerThrLeu                               265270275280                                                                   GAAGAAAAAGAATACCCTGGAGCTCACACAACAACAATTAAGTATACT915                            GluGluLysGluTyrProGlyAlaHisThrThrThrIleLysTyrThr                               285290295                                                                      TCTGCTCTGACCTATGATGCCGTTCAAGTGATGACTGAAGCCTTCCGC963                            SerAlaLeuThrTyrAspAlaValGlnValMetThrGluAlaPheArg                               300305310                                                                      AACCTAAGGAAGCAAAGAATTGAAATCTCCCGAAGGGGGAATGCAGGA1011                           AsnLeuArgLysGlnArgIleGluIleSerArgArgGlyAsnAlaGly                               315320325                                                                      GACTGTCTGGCAAACCCAGCAGTGCCCTGGGGACAAGGTGTAGAAATA1059                           AspCysLeuAlaAsnProAlaValProTrpGlyGlnGlyValGluIle                               330335340                                                                      GAAAGGGCCCTCAAACAGGTTCAGGTTGAAGGTCTCTCAGGAAATATA1107                           GluArgAlaLeuLysGlnValGlnValGluGlyLeuSerGlyAsnIle                               345350355360                                                                   AAGTTTGACCAGAATGGAAAAAGAATAAACTATACAATTAACATCATG1155                           LysPheAspGlnAsnGlyLysArgIleAsnTyrThrIleAsnIleMet                               365370375                                                                      GAGCTCAAAACTAATGGGCCCCGGAAGATTGGCTACTGGAGTGAAGTG1203                           GluLeuLysThrAsnGlyProArgLysIleGlyTyrTrpSerGluVal                               380385390                                                                      GACAAAATGGTTGTTACCCTTACTGAGCTCCCTTCTGGAAATGACACC1251                           AspLysMetValValThrLeuThrGluLeuProSerGlyAsnAspThr                               395400405                                                                      TCTGGGCTTGAGAATAAGACTGTTGTTGTCACCACAATTTTGGAATCT1299                           SerGlyLeuGluAsnLysThrValValValThrThrIleLeuGluSer                               410415420                                                                      CCGTATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGCAATGAG1347                           ProTyrValMetMetLysLysAsnHisGluMetLeuGluGlyAsnGlu                               425430435440                                                                   CGCTATGAGGGCTACTGTGTTGACCTGGCTGCAGAAATCGCCAAACAT1395                           ArgTyrGluGlyTyrCysValAspLeuAlaAlaGluIleAlaLysHis                               445450455                                                                      TGTGGGTTCAAGTACAAGTTGACAATTGTTGGTGATGGCAAGTATGGG1443                           CysGlyPheLysTyrLysLeuThrIleValGlyAspGlyLysTyrGly                               460465470                                                                      GCCAGGGATGCAGACACGAAAATTTGGAATGGGATGGTTGGAGAACTT1491                           AlaArgAspAlaAspThrLysIleTrpAsnGlyMetValGlyGluLeu                               475480485                                                                      GTATATGGGAAAGCTGATATTGCAATTGCTCCATTAACTATTACCCTT1539                           ValTyrGlyLysAlaAspIleAlaIleAlaProLeuThrIleThrLeu                               490495500                                                                      GTGAGAGAAGAGGTGATTGACTTCTCAAAGCCCTTCATGAGCCTCGGG1587                           ValArgGluGluValIleAspPheSerLysProPheMetSerLeuGly                               505510515520                                                                   ATATCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTT1635                           IleSerIleMetIleLysLysProGlnLysSerLysProGlyValPhe                               525530535                                                                      TCCTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTTTTT1683                           SerPheLeuAspProLeuAlaTyrGluIleTrpMetCysIleValPhe                               540545550                                                                      GCCTACATTGGGGTCAGTGTAGTTTTATTCCTGGTCAGCAGATTTAGC1731                           AlaTyrIleGlyValSerValValLeuPheLeuValSerArgPheSer                               555560565                                                                      CCCTACGAGTGGCACACTGAGGAGTTTGAAGATGGAAGAGAAACACAA1779                           ProTyrGluTrpHisThrGluGluPheGluAspGlyArgGluThrGln                               570575580                                                                      AGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTT1827                           SerSerGluSerThrAsnGluPheGlyIlePheAsnSerLeuTrpPhe                               585590595600                                                                   TCCTTGGGTGCCTTTATGCGGCAAGGATGCGATATTTCGCCAAGATCC1875                           SerLeuGlyAlaPheMetArgGlnGlyCysAspIleSerProArgSer                               605610615                                                                      CTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACCCTGATC1923                           LeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeuIle                               620625630                                                                      ATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAG1971                           IleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrValGlu                               635640645                                                                      AGGATGGTGTCTCCCATCGAAAGTGCTGAGGATCTTTCTAAGCAAACA2019                           ArgMetValSerProIleGluSerAlaGluAspLeuSerLysGlnThr                               650655660                                                                      GAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAAAGAGTTTTTC2067                           GluIleAlaTyrGlyThrLeuAspSerGlySerThrLysGluPhePhe                               665670675680                                                                   AGGAGATCTAAAATTGCAGTGTTTGATAAAATGTGGACCTACATGCGG2115                           ArgArgSerLysIleAlaValPheAspLysMetTrpThrTyrMetArg                               685690695                                                                      AGTGCGGAGCCCTCTGTGTTTGTGAGGACTACGGCCGAAGGGGTGGCT2163                           SerAlaGluProSerValPheValArgThrThrAlaGluGlyValAla                               700705710                                                                      AGAGTGCGGAAGTCCAAAGGGAAATATGCCTACTTGTTGGAGTCCACG2211                           ArgValArgLysSerLysGlyLysTyrAlaTyrLeuLeuGluSerThr                               715720725                                                                      ATGAACGAGTACATTGAGCAAAGGAAGCCTTGCGACACCATGAAAGTT2259                           MetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLysVal                               730735740                                                                      GGTGGAAACCTGGATTCCAAAGGCTATGGCATCGCAACACCTAAAGGA2307                           GlyGlyAsnLeuAspSerLysGlyTyrGlyIleAlaThrProLysGly                               745750755760                                                                   TCCTCATTAAGAAATGCGGTTAACCTCGCAGTACTAAAACTGAATGAA2355                           SerSerLeuArgAsnAlaValAsnLeuAlaValLeuLysLeuAsnGlu                               765770775                                                                      CAAGGCCTGTTGGACAAATTGAAAAACAAATGGTGGTACGACAAAGGA2403                           GlnGlyLeuLeuAspLysLeuLysAsnLysTrpTrpTyrAspLysGly                               780785790                                                                      GAGTGCGGCAGCGGGGGAGGTGATTCCAAGGAAAAGACCAGTGCCCTC2451                           GluCysGlySerGlyGlyGlyAspSerLysGluLysThrSerAlaLeu                               795800805                                                                      AGTCTGAGCAACGTTGCTGGAGTATTCTACATCCTTGTCGGGGGCCTT2499                           SerLeuSerAsnValAlaGlyValPheTyrIleLeuValGlyGlyLeu                               810815820                                                                      GGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGG2547                           GlyLeuAlaMetLeuValAlaLeuIleGluPheCysTyrLysSerArg                               825830835840                                                                   GCCGAGGCGAAACGAATGAAGGTGGCAAAGAATGCACAGAATATTAAC2595                           AlaGluAlaLysArgMetLysValAlaLysAsnAlaGlnAsnIleAsn                               845850855                                                                      CCATCTTCCTCGCAGAATTCACAGAATTTTGCAACTTATAAGGAAGGT2643                           ProSerSerSerGlnAsnSerGlnAsnPheAlaThrTyrLysGluGly                               860865870                                                                      TACAACGTATATGGCATCGAAAGTGTTAAAATTTAGGGGATGACCTTGAAATG2696                      TyrAsnValTyrGlyIleGluSerValLysIle                                              875880                                                                         ATGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTACTGGAGAAAATGGACGTG2756               TTATGACTCCAGAATTTCCCAAAGCAGTGCATGCTGTCCCTTACGTGAGTCCTGGCATGG2816               GAATGAATGTCAGTGTGACTGATCTCTCGTGATTGATAAGAACCTTTTGAGTGCCTTACA2876               CAATGGTTTTCTTGTGTTTATTGTCAAAGTGGTGAGAGGCATCCAGTATCTTGAAGACTT2936               TTCTTTCAGCCAAGAATTC2955                                                        (2) INFORMATION FOR SEQ ID NO:8:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 883 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:                                       MetGlnLysIleMetHisValSerValLeuLeuSerProValLeuTrp                               151015                                                                         GlyLeuIlePheGlyValSerSerAsnSerIleGlnIleGlyGlyLeu                               202530                                                                         PheProArgGlyAlaAspGlnGluTyrSerAlaPheArgValGlyMet                               354045                                                                         ValGlnPheSerThrSerGluPheArgLeuThrProHisIleAspAsn                               505560                                                                         LeuGluValAlaAsnSerPheAlaValThrAsnAlaPheCysSerGln                               65707580                                                                       PheSerArgGlyValTyrAlaIlePheGlyPheTyrAspLysLysSer                               859095                                                                         ValAsnThrIleThrSerPheCysGlyThrLeuHisValSerPheIle                               100105110                                                                      ThrProSerPheProThrAspGlyThrHisProPheValIleGlnMet                               115120125                                                                      ArgProAspLeuLysGlyAlaLeuLeuSerLeuIleGluTyrTyrGln                               130135140                                                                      TrpAspLysPheAlaTyrLeuTyrAspSerAspArgGlyLeuSerThr                               145150155160                                                                   LeuGlnAlaValLeuAspSerAlaAlaGluLysLysTrpGlnValThr                               165170175                                                                      AlaIleAsnValGlyAsnIleAsnAsnAspLysLysAspGluMetTyr                               180185190                                                                      ArgSerLeuPheGlnAspLeuGluLeuLysLysGluArgArgValIle                               195200205                                                                      LeuAspCysGluArgAspLysValAsnAspIleValAspGlnValIle                               210215220                                                                      ThrIleGlyLysHisValLysGlyTyrHisTyrIleIleAlaAsnLeu                               225230235240                                                                   GlyPheThrAspGlyAspLeuLeuLysIleGlnPheGlyGlyAlaAsn                               245250255                                                                      ValSerGlyPheGlnIleValAspTyrAspAspSerLeuValSerLys                               260265270                                                                      PheIleGluArgTrpSerThrLeuGluGluLysGluTyrProGlyAla                               275280285                                                                      HisThrThrThrIleLysTyrThrSerAlaLeuThrTyrAspAlaVal                               290295300                                                                      GlnValMetThrGluAlaPheArgAsnLeuArgLysGlnArgIleGlu                               305310315320                                                                   IleSerArgArgGlyAsnAlaGlyAspCysLeuAlaAsnProAlaVal                               325330335                                                                      ProTrpGlyGlnGlyValGluIleGluArgAlaLeuLysGlnValGln                               340345350                                                                      ValGluGlyLeuSerGlyAsnIleLysPheAspGlnAsnGlyLysArg                               355360365                                                                      IleAsnTyrThrIleAsnIleMetGluLeuLysThrAsnGlyProArg                               370375380                                                                      LysIleGlyTyrTrpSerGluValAspLysMetValValThrLeuThr                               385390395400                                                                   GluLeuProSerGlyAsnAspThrSerGlyLeuGluAsnLysThrVal                               405410415                                                                      ValValThrThrIleLeuGluSerProTyrValMetMetLysLysAsn                               420425430                                                                      HisGluMetLeuGluGlyAsnGluArgTyrGluGlyTyrCysValAsp                               435440445                                                                      LeuAlaAlaGluIleAlaLysHisCysGlyPheLysTyrLysLeuThr                               450455460                                                                      IleValGlyAspGlyLysTyrGlyAlaArgAspAlaAspThrLysIle                               465470475480                                                                   TrpAsnGlyMetValGlyGluLeuValTyrGlyLysAlaAspIleAla                               485490495                                                                      IleAlaProLeuThrIleThrLeuValArgGluGluValIleAspPhe                               500505510                                                                      SerLysProPheMetSerLeuGlyIleSerIleMetIleLysLysPro                               515520525                                                                      GlnLysSerLysProGlyValPheSerPheLeuAspProLeuAlaTyr                               530535540                                                                      GluIleTrpMetCysIleValPheAlaTyrIleGlyValSerValVal                               545550555560                                                                   LeuPheLeuValSerArgPheSerProTyrGluTrpHisThrGluGlu                               565570575                                                                      PheGluAspGlyArgGluThrGlnSerSerGluSerThrAsnGluPhe                               580585590                                                                      GlyIlePheAsnSerLeuTrpPheSerLeuGlyAlaPheMetArgGln                               595600605                                                                      GlyCysAspIleSerProArgSerLeuSerGlyArgIleValGlyGly                               610615620                                                                      ValTrpTrpPhePheThrLeuIleIleIleSerSerTyrThrAlaAsn                               625630635640                                                                   LeuAlaAlaPheLeuThrValGluArgMetValSerProIleGluSer                               645650655                                                                      AlaGluAspLeuSerLysGlnThrGluIleAlaTyrGlyThrLeuAsp                               660665670                                                                      SerGlySerThrLysGluPhePheArgArgSerLysIleAlaValPhe                               675680685                                                                      AspLysMetTrpThrTyrMetArgSerAlaGluProSerValPheVal                               690695700                                                                      ArgThrThrAlaGluGlyValAlaArgValArgLysSerLysGlyLys                               705710715720                                                                   TyrAlaTyrLeuLeuGluSerThrMetAsnGluTyrIleGluGlnArg                               725730735                                                                      LysProCysAspThrMetLysValGlyGlyAsnLeuAspSerLysGly                               740745750                                                                      TyrGlyIleAlaThrProLysGlySerSerLeuArgAsnAlaValAsn                               755760765                                                                      LeuAlaValLeuLysLeuAsnGluGlnGlyLeuLeuAspLysLeuLys                               770775780                                                                      AsnLysTrpTrpTyrAspLysGlyGluCysGlySerGlyGlyGlyAsp                               785790795800                                                                   SerLysGluLysThrSerAlaLeuSerLeuSerAsnValAlaGlyVal                               805810815                                                                      PheTyrIleLeuValGlyGlyLeuGlyLeuAlaMetLeuValAlaLeu                               820825830                                                                      IleGluPheCysTyrLysSerArgAlaGluAlaLysArgMetLysVal                               835840845                                                                      AlaLysAsnAlaGlnAsnIleAsnProSerSerSerGlnAsnSerGln                               850855860                                                                      AsnPheAlaThrTyrLysGluGlyTyrAsnValTyrGlyIleGluSer                               865870875880                                                                   ValLysIle                                                                      (2) INFORMATION FOR SEQ ID NO: 9:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2989 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: adult                                                 (C) TISSUE TYPE: brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 73..2736                                                         (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9:                                       CTGACGACTCCTGAGTTGCGCCCATGCTCTTGTCAGCTTCGTTTTAGGCGTAGCATGGCC60                 AGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTA108                            MetGlyGlnSerValLeuArgAlaValPhePheLeu                                           1510                                                                           GTCCTGGGGCTTTTGGGTCATTCTCACGGAGGATTCCCCAACACCATC156                            ValLeuGlyLeuLeuGlyHisSerHisGlyGlyPheProAsnThrIle                               152025                                                                         AGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGAGCACAGCGCT204                            SerIleGlyGlyLeuPheMetArgAsnThrValGlnGluHisSerAla                               303540                                                                         TTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAG252                            PheArgPheAlaValGlnLeuTyrAsnThrAsnGlnAsnThrThrGlu                               45505560                                                                       AAGCCCTTCCATTTGAATTACCACGTAGATCACTTGGATTCCTCCAAT300                            LysProPheHisLeuAsnTyrHisValAspHisLeuAspSerSerAsn                               657075                                                                         AGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGTTCTCGAGAGGGGTG348                            SerPheSerValThrAsnAlaPheCysSerGlnPheSerArgGlyVal                               808590                                                                         TATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACC396                            TyrAlaIlePheGlyPheTyrAspGlnMetSerMetAsnThrLeuThr                               95100105                                                                       TCCTTCTGTGGGGCCCTGCACACATCCTTTGTTACGCCTAGCTTCCCC444                            SerPheCysGlyAlaLeuHisThrSerPheValThrProSerPhePro                               110115120                                                                      ACTGACGCAGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTGAAG492                            ThrAspAlaAspValGlnPheValIleGlnMetArgProAlaLeuLys                               125130135140                                                                   GGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTG540                            GlyAlaIleLeuSerLeuLeuGlyHisTyrLysTrpGluLysPheVal                               145150155                                                                      TACCTCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATG588                            TyrLeuTyrAspThrGluArgGlyPheSerIleLeuGlnAlaIleMet                               160165170                                                                      GAAGCAGCAGTGCAAAACAACTGGCAAGTAACAGCAAGGTCTGTGGGA636                            GluAlaAlaValGlnAsnAsnTrpGlnValThrAlaArgSerValGly                               175180185                                                                      AACATAAAGGACGTCCAAGAATTCAGGCGCATCATTGAAGAAATGGAC684                            AsnIleLysAspValGlnGluPheArgArgIleIleGluGluMetAsp                               190195200                                                                      AGGAGGCAGGAAAAGCGATACTTGATTGACTGCGAAGTCGAAAGGATT732                            ArgArgGlnGluLysArgTyrLeuIleAspCysGluValGluArgIle                               205210215220                                                                   AACACAATTTTGGAACAGGTTGTGATCCTAGGGAAACACTCAAGAGGT780                            AsnThrIleLeuGluGlnValValIleLeuGlyLysHisSerArgGly                               225230235                                                                      TATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACTGGAA828                            TyrHisTyrMetLeuAlaAsnLeuGlyPheThrAspIleLeuLeuGlu                               240245250                                                                      AGAGTCATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAAC876                            ArgValMetHisGlyGlyAlaAsnIleThrGlyPheGlnIleValAsn                               255260265                                                                      AATGAAAACCCTATGGTTCAGCAGTTCATACAGCGCTGGGTGAGGCTG924                            AsnGluAsnProMetValGlnGlnPheIleGlnArgTrpValArgLeu                               270275280                                                                      GATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAAAGTATACA972                            AspGluArgGluPheProGluAlaLysAsnAlaProLeuLysTyrThr                               285290295300                                                                   TCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCTTTCCGC1020                           SerAlaLeuThrHisAspAlaIleLeuValIleAlaGluAlaPheArg                               305310315                                                                      TACCTGAGGAGGCAGCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGA1068                           TyrLeuArgArgGlnArgValAspValSerArgArgGlySerAlaGly                               320325330                                                                      GACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATT1116                           AspCysLeuAlaAsnProAlaValProTrpSerGlnGlyIleAspIle                               335340345                                                                      GAGAGAGCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAATATT1164                           GluArgAlaLeuLysMetValGlnValGlnGlyMetThrGlyAsnIle                               350355360                                                                      CAATTTGACACTTATGGACGTAGGACAAATTATACCATCGATGTGTAT1212                           GlnPheAspThrTyrGlyArgArgThrAsnTyrThrIleAspValTyr                               365370375380                                                                   GAAATGAAAGTCAGTGGCTCTCGAAAAGCTGGCTACTGGAACGAGTAT1260                           GluMetLysValSerGlySerArgLysAlaGlyTyrTrpAsnGluTyr                               385390395                                                                      GAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCA1308                           GluArgPheValProPheSerAspGlnGlnIleSerAsnAspSerAla                               400405410                                                                      TCCTCAGAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCA1356                           SerSerGluAsnArgThrIleValValThrThrIleLeuGluSerPro                               415420425                                                                      TATGTAATGTACAAGAAGAACCATGAGCAACTGGAAGGAAATGAACGA1404                           TyrValMetTyrLysLysAsnHisGluGlnLeuGluGlyAsnGluArg                               430435440                                                                      TATGAAGGCTATTGTGTAGACCTAGCCTATGAAATAGCCAAACATGTA1452                           TyrGluGlyTyrCysValAspLeuAlaTyrGluIleAlaLysHisVal                               445450455460                                                                   AGGATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATATGGTGCA1500                           ArgIleLysTyrLysLeuSerIleValGlyAspGlyLysTyrGlyAla                               465470475                                                                      AGGGATCCAGAGACTAAAATATGGAACGGCATGGTTGGGGAACTTGTC1548                           ArgAspProGluThrLysIleTrpAsnGlyMetValGlyGluLeuVal                               480485490                                                                      TATGGGAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTC1596                           TyrGlyArgAlaAspIleAlaValAlaProLeuThrIleThrLeuVal                               495500505                                                                      CGTGAAGAAGTCATAGATTTTTCAAAGCCATTCATGAGCCTGGGCATC1644                           ArgGluGluValIleAspPheSerLysProPheMetSerLeuGlyIle                               510515520                                                                      TCCATCATGATAAAGAAGCCTCAGAAATCAAAACCAGGCGTATTCTCA1692                           SerIleMetIleLysLysProGlnLysSerLysProGlyValPheSer                               525530535540                                                                   TTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTCTTTGCT1740                           PheLeuAspProLeuAlaTyrGluIleTrpMetCysIleValPheAla                               545550555                                                                      TACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCT1788                           TyrIleGlyValSerValValLeuPheLeuValSerArgPheSerPro                               560565570                                                                      TATGAATGGCACTTGGAAGACAACAATGAAGAACCTCGTGACCCACAA1836                           TyrGluTrpHisLeuGluAspAsnAsnGluGluProArgAspProGln                               575580585                                                                      AGTCCTCCTGATCCTCCAAATGAATTTGGAATATTTAACAGTCTTTGG1884                           SerProProAspProProAsnGluPheGlyIlePheAsnSerLeuTrp                               590595600                                                                      TTTTCCTTGGGTGCCTTTATGCAGCAAGGATGTGATATTTCTCCAAGA1932                           PheSerLeuGlyAlaPheMetGlnGlnGlyCysAspIleSerProArg                               605610615620                                                                   TCACTCTCCGGGCGCATTGTTGGAGGGGTTTGGTGGTTCTTCACCCTG1980                           SerLeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeu                               625630635                                                                      ATCATAATTTCTTCCTATACTGCCAATCTCGCTGCTTTCCTGACTGTG2028                           IleIleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrVal                               640645650                                                                      GAGAGGATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAG2076                           GluArgMetValSerProIleGluSerAlaGluAspLeuAlaLysGln                               655660665                                                                      ACTGAAATTGCATATGGGACCCTGGACTCCGGTTCAACAAAAGAATTT2124                           ThrGluIleAlaTyrGlyThrLeuAspSerGlySerThrLysGluPhe                               670675680                                                                      TTCAGAAGATCCAAAATTGCTGTGTACGAGAAAATGTGGTCTTACATG2172                           PheArgArgSerLysIleAlaValTyrGluLysMetTrpSerTyrMet                               685690695700                                                                   AAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGACGGAGTG2220                           LysSerAlaGluProSerValPheThrLysThrThrAlaAspGlyVal                               705710715                                                                      GCCCGAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCA2268                           AlaArgValArgLysSerLysGlyLysPheAlaPheLeuLeuGluSer                               720725730                                                                      ACCATGAATGAGTACATTGAGCAGAGAAAACCATGTGATACGATGAAA2316                           ThrMetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLys                               735740745                                                                      GTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAA2364                           ValGlyGlyAsnLeuAspSerLysGlyTyrGlyValAlaThrProLys                               750755760                                                                      GGCTCAGCATTAGGAACGCCTGTAAACCTTGCAGTATTGAAACTCAGT2412                           GlySerAlaLeuGlyThrProValAsnLeuAlaValLeuLysLeuSer                               765770775780                                                                   GAACAAGGCATCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAG2460                           GluGlnGlyIleLeuAspLysLeuLysAsnLysTrpTrpTyrAspLys                               785790795                                                                      GGGGAATGTGGAGCCAAGGACTCCGGGAGTAAGGACAAGACCAGCGCT2508                           GlyGluCysGlyAlaLysAspSerGlySerLysAspLysThrSerAla                               800805810                                                                      CTGAGCCTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGT2556                           LeuSerLeuSerAsnValAlaGlyValPheTyrIleLeuValGlyGly                               815820825                                                                      CTGGGGCTGGCCATGATGGTGGCTTTGATAGAATTCTGTTACAAATCA2604                           LeuGlyLeuAlaMetMetValAlaLeuIleGluPheCysTyrLysSer                               830835840                                                                      CGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTT2652                           ArgAlaGluSerLysArgMetLysLeuThrLysAsnThrGlnAsnPhe                               845850855860                                                                   AAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATACAGAGAA2700                           LysProAlaProAlaThrAsnThrGlnAsnTyrAlaThrTyrArgGlu                               865870875                                                                      GGCTACAACGTGTATGGAACAGAGAGTGTTAAGATCTAGGGATCCC2746                             GlyTyrAsnValTyrGlyThrGluSerValLysIle                                           880885                                                                         TTCCCACTGGAGGCATGTGATGAGAGGAAATCACCGAAAACGTGGCTGCTTCAAGGATCC2806               TGAGCCAGATTTCACTCTCCTTGGTGTCGGGCATGACACGAATATTGCTGATGGTGCAAT2866               GACCTTTCAATAGGAAAAACTGGTTTTTTTTTCCTTCAGTGCCTTATGGAACACTCTGAG2926               ACTCGCGACAATGCAAACCATCATTGAAATCTTTTTGCTTTGCTTGAAAAAAAAAAAAAA2986               AAA2989                                                                        (2) INFORMATION FOR SEQ ID NO:10:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 888 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:                                      MetGlyGlnSerValLeuArgAlaValPhePheLeuValLeuGlyLeu                               151015                                                                         LeuGlyHisSerHisGlyGlyPheProAsnThrIleSerIleGlyGly                               202530                                                                         LeuPheMetArgAsnThrValGlnGluHisSerAlaPheArgPheAla                               354045                                                                         ValGlnLeuTyrAsnThrAsnGlnAsnThrThrGluLysProPheHis                               505560                                                                         LeuAsnTyrHisValAspHisLeuAspSerSerAsnSerPheSerVal                               65707580                                                                       ThrAsnAlaPheCysSerGlnPheSerArgGlyValTyrAlaIlePhe                               859095                                                                         GlyPheTyrAspGlnMetSerMetAsnThrLeuThrSerPheCysGly                               100105110                                                                      AlaLeuHisThrSerPheValThrProSerPheProThrAspAlaAsp                               115120125                                                                      ValGlnPheValIleGlnMetArgProAlaLeuLysGlyAlaIleLeu                               130135140                                                                      SerLeuLeuGlyHisTyrLysTrpGluLysPheValTyrLeuTyrAsp                               145150155160                                                                   ThrGluArgGlyPheSerIleLeuGlnAlaIleMetGluAlaAlaVal                               165170175                                                                      GlnAsnAsnTrpGlnValThrAlaArgSerValGlyAsnIleLysAsp                               180185190                                                                      ValGlnGluPheArgArgIleIleGluGluMetAspArgArgGlnGlu                               195200205                                                                      LysArgTyrLeuIleAspCysGluValGluArgIleAsnThrIleLeu                               210215220                                                                      GluGlnValValIleLeuGlyLysHisSerArgGlyTyrHisTyrMet                               225230235240                                                                   LeuAlaAsnLeuGlyPheThrAspIleLeuLeuGluArgValMetHis                               245250255                                                                      GlyGlyAlaAsnIleThrGlyPheGlnIleValAsnAsnGluAsnPro                               260265270                                                                      MetValGlnGlnPheIleGlnArgTrpValArgLeuAspGluArgGlu                               275280285                                                                      PheProGluAlaLysAsnAlaProLeuLysTyrThrSerAlaLeuThr                               290295300                                                                      HisAspAlaIleLeuValIleAlaGluAlaPheArgTyrLeuArgArg                               305310315320                                                                   GlnArgValAspValSerArgArgGlySerAlaGlyAspCysLeuAla                               325330335                                                                      AsnProAlaValProTrpSerGlnGlyIleAspIleGluArgAlaLeu                               340345350                                                                      LysMetValGlnValGlnGlyMetThrGlyAsnIleGlnPheAspThr                               355360365                                                                      TyrGlyArgArgThrAsnTyrThrIleAspValTyrGluMetLysVal                               370375380                                                                      SerGlySerArgLysAlaGlyTyrTrpAsnGluTyrGluArgPheVal                               385390395400                                                                   ProPheSerAspGlnGlnIleSerAsnAspSerAlaSerSerGluAsn                               405410415                                                                      ArgThrIleValValThrThrIleLeuGluSerProTyrValMetTyr                               420425430                                                                      LysLysAsnHisGluGlnLeuGluGlyAsnGluArgTyrGluGlyTyr                               435440445                                                                      CysValAspLeuAlaTyrGluIleAlaLysHisValArgIleLysTyr                               450455460                                                                      LysLeuSerIleValGlyAspGlyLysTyrGlyAlaArgAspProGlu                               465470475480                                                                   ThrLysIleTrpAsnGlyMetValGlyGluLeuValTyrGlyArgAla                               485490495                                                                      AspIleAlaValAlaProLeuThrIleThrLeuValArgGluGluVal                               500505510                                                                      IleAspPheSerLysProPheMetSerLeuGlyIleSerIleMetIle                               515520525                                                                      LysLysProGlnLysSerLysProGlyValPheSerPheLeuAspPro                               530535540                                                                      LeuAlaTyrGluIleTrpMetCysIleValPheAlaTyrIleGlyVal                               545550555560                                                                   SerValValLeuPheLeuValSerArgPheSerProTyrGluTrpHis                               565570575                                                                      LeuGluAspAsnAsnGluGluProArgAspProGlnSerProProAsp                               580585590                                                                      ProProAsnGluPheGlyIlePheAsnSerLeuTrpPheSerLeuGly                               595600605                                                                      AlaPheMetGlnGlnGlyCysAspIleSerProArgSerLeuSerGly                               610615620                                                                      ArgIleValGlyGlyValTrpTrpPhePheThrLeuIleIleIleSer                               625630635640                                                                   SerTyrThrAlaAsnLeuAlaAlaPheLeuThrValGluArgMetVal                               645650655                                                                      SerProIleGluSerAlaGluAspLeuAlaLysGlnThrGluIleAla                               660665670                                                                      TyrGlyThrLeuAspSerGlySerThrLysGluPhePheArgArgSer                               675680685                                                                      LysIleAlaValTyrGluLysMetTrpSerTyrMetLysSerAlaGlu                               690695700                                                                      ProSerValPheThrLysThrThrAlaAspGlyValAlaArgValArg                               705710715720                                                                   LysSerLysGlyLysPheAlaPheLeuLeuGluSerThrMetAsnGlu                               725730735                                                                      TyrIleGluGlnArgLysProCysAspThrMetLysValGlyGlyAsn                               740745750                                                                      LeuAspSerLysGlyTyrGlyValAlaThrProLysGlySerAlaLeu                               755760765                                                                      GlyThrProValAsnLeuAlaValLeuLysLeuSerGluGlnGlyIle                               770775780                                                                      LeuAspLysLeuLysAsnLysTrpTrpTyrAspLysGlyGluCysGly                               785790795800                                                                   AlaLysAspSerGlySerLysAspLysThrSerAlaLeuSerLeuSer                               805810815                                                                      AsnValAlaGlyValPheTyrIleLeuValGlyGlyLeuGlyLeuAla                               820825830                                                                      MetMetValAlaLeuIleGluPheCysTyrLysSerArgAlaGluSer                               835840845                                                                      LysArgMetLysLeuThrLysAsnThrGlnAsnPheLysProAlaPro                               850855860                                                                      AlaThrAsnThrGlnAsnTyrAlaThrTyrArgGluGlyTyrAsnVal                               865870875880                                                                   TyrGlyThrGluSerValLysIle                                                       885                                                                            (2) INFORMATION FOR SEQ ID NO: 11:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2989 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: adult                                                 (C) TISSUE TYPE: brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 73..2736                                                         (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:                                      CTGACGACTCCTGAGTTGCGCCCATGCTCTTGTCAGCTTCGTTTTAGGCGTAGCATGGCC60                 AGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTA108                            MetGlyGlnSerValLeuArgAlaValPhePheLeu                                           1510                                                                           GTCCTGGGGCTTTTGGGTCATTCTCACGGAGGATTCCCCAACACCATC156                            ValLeuGlyLeuLeuGlyHisSerHisGlyGlyPheProAsnThrIle                               152025                                                                         AGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGAGCACAGCGCT204                            SerIleGlyGlyLeuPheMetArgAsnThrValGlnGluHisSerAla                               303540                                                                         TTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAG252                            PheArgPheAlaValGlnLeuTyrAsnThrAsnGlnAsnThrThrGlu                               45505560                                                                       AAGCCCTTCCATTTGAATTACCACGTAGATCACTTGGATTCCTCCAAT300                            LysProPheHisLeuAsnTyrHisValAspHisLeuAspSerSerAsn                               657075                                                                         AGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGTTCTCGAGAGGGGTG348                            SerPheSerValThrAsnAlaPheCysSerGlnPheSerArgGlyVal                               808590                                                                         TATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACC396                            TyrAlaIlePheGlyPheTyrAspGlnMetSerMetAsnThrLeuThr                               95100105                                                                       TCCTTCTGTGGGGCCCTGCACACATCCTTTGTTACGCCTAGCTTCCCC444                            SerPheCysGlyAlaLeuHisThrSerPheValThrProSerPhePro                               110115120                                                                      ACTGACGCAGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTGAAG492                            ThrAspAlaAspValGlnPheValIleGlnMetArgProAlaLeuLys                               125130135140                                                                   GGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTG540                            GlyAlaIleLeuSerLeuLeuGlyHisTyrLysTrpGluLysPheVal                               145150155                                                                      TACCTCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATG588                            TyrLeuTyrAspThrGluArgGlyPheSerIleLeuGlnAlaIleMet                               160165170                                                                      GAAGCAGCAGTGCAAAACAACTGGCAAGTAACAGCAAGGTCTGTGGGA636                            GluAlaAlaValGlnAsnAsnTrpGlnValThrAlaArgSerValGly                               175180185                                                                      AACATAAAGGACGTCCAAGAATTCAGGCGCATCATTGAAGAAATGGAC684                            AsnIleLysAspValGlnGluPheArgArgIleIleGluGluMetAsp                               190195200                                                                      AGGAGGCAGGAAAAGCGATACTTGATTGACTGCGAAGTCGAAAGGATT732                            ArgArgGlnGluLysArgTyrLeuIleAspCysGluValGluArgIle                               205210215220                                                                   AACACAATTTTGGAACAGGTTGTGATCCTAGGGAAACACTCAAGAGGT780                            AsnThrIleLeuGluGlnValValIleLeuGlyLysHisSerArgGly                               225230235                                                                      TATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACTGGAA828                            TyrHisTyrMetLeuAlaAsnLeuGlyPheThrAspIleLeuLeuGlu                               240245250                                                                      AGAGTCATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAAC876                            ArgValMetHisGlyGlyAlaAsnIleThrGlyPheGlnIleValAsn                               255260265                                                                      AATGAAAACCCTATGGTTCAGCAGTTCATACAGCGCTGGGTGAGGCTG924                            AsnGluAsnProMetValGlnGlnPheIleGlnArgTrpValArgLeu                               270275280                                                                      GATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAAAGTATACA972                            AspGluArgGluPheProGluAlaLysAsnAlaProLeuLysTyrThr                               285290295300                                                                   TCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCTTTCCGC1020                           SerAlaLeuThrHisAspAlaIleLeuValIleAlaGluAlaPheArg                               305310315                                                                      TACCTGAGGAGGCAGCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGA1068                           TyrLeuArgArgGlnArgValAspValSerArgArgGlySerAlaGly                               320325330                                                                      GACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATT1116                           AspCysLeuAlaAsnProAlaValProTrpSerGlnGlyIleAspIle                               335340345                                                                      GAGAGAGCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAATATT1164                           GluArgAlaLeuLysMetValGlnValGlnGlyMetThrGlyAsnIle                               350355360                                                                      CAATTTGACACTTATGGACGTAGGACAAATTATACCATCGATGTGTAT1212                           GlnPheAspThrTyrGlyArgArgThrAsnTyrThrIleAspValTyr                               365370375380                                                                   GAAATGAAAGTCAGTGGCTCTCGAAAAGCTGGCTACTGGAACGAGTAT1260                           GluMetLysValSerGlySerArgLysAlaGlyTyrTrpAsnGluTyr                               385390395                                                                      GAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCA1308                           GluArgPheValProPheSerAspGlnGlnIleSerAsnAspSerAla                               400405410                                                                      TCCTCAGAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCA1356                           SerSerGluAsnArgThrIleValValThrThrIleLeuGluSerPro                               415420425                                                                      TATGTAATGTACAAGAAGAACCATGAGCAACTGGAAGGAAATGAACGA1404                           TyrValMetTyrLysLysAsnHisGluGlnLeuGluGlyAsnGluArg                               430435440                                                                      TATGAAGGCTATTGTGTAGACCTAGCCTATGAAATAGCCAAACATGTA1452                           TyrGluGlyTyrCysValAspLeuAlaTyrGluIleAlaLysHisVal                               445450455460                                                                   AGGATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATATGGTGCA1500                           ArgIleLysTyrLysLeuSerIleValGlyAspGlyLysTyrGlyAla                               465470475                                                                      AGGGATCCAGAGACTAAAATATGGAACGGCATGGTTGGGGAACTTGTC1548                           ArgAspProGluThrLysIleTrpAsnGlyMetValGlyGluLeuVal                               480485490                                                                      TATGGGAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTC1596                           TyrGlyArgAlaAspIleAlaValAlaProLeuThrIleThrLeuVal                               495500505                                                                      CGTGAAGAAGTCATAGATTTTTCAAAGCCATTCATGAGCCTGGGCATC1644                           ArgGluGluValIleAspPheSerLysProPheMetSerLeuGlyIle                               510515520                                                                      TCCATCATGATAAAGAAGCCTCAGAAATCAAAACCAGGCGTATTCTCA1692                           SerIleMetIleLysLysProGlnLysSerLysProGlyValPheSer                               525530535540                                                                   TTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTCTTTGCT1740                           PheLeuAspProLeuAlaTyrGluIleTrpMetCysIleValPheAla                               545550555                                                                      TACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCT1788                           TyrIleGlyValSerValValLeuPheLeuValSerArgPheSerPro                               560565570                                                                      TATGAATGGCACTTGGAAGACAACAATGAAGAACCTCGTGACCCACAA1836                           TyrGluTrpHisLeuGluAspAsnAsnGluGluProArgAspProGln                               575580585                                                                      AGTCCTCCTGATCCTCCAAATGAATTTGGAATATTTAACAGTCTTTGG1884                           SerProProAspProProAsnGluPheGlyIlePheAsnSerLeuTrp                               590595600                                                                      TTTTCCTTGGGTGCCTTTATGCAGCAAGGATGTGATATTTCTCCAAGA1932                           PheSerLeuGlyAlaPheMetGlnGlnGlyCysAspIleSerProArg                               605610615620                                                                   TCACTCTCCGGGCGCATTGTTGGAGGGGTTTGGTGGTTCTTCACCCTG1980                           SerLeuSerGlyArgIleValGlyGlyValTrpTrpPhePheThrLeu                               625630635                                                                      ATCATAATTTCTTCCTATACTGCCAATCTCGCTGCTTTCCTGACTGTG2028                           IleIleIleSerSerTyrThrAlaAsnLeuAlaAlaPheLeuThrVal                               640645650                                                                      GAGAGGATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAG2076                           GluArgMetValSerProIleGluSerAlaGluAspLeuAlaLysGln                               655660665                                                                      ACTGAAATTGCATATGGGACCCTGGACTCCGGTTCAACAAAAGAATTT2124                           ThrGluIleAlaTyrGlyThrLeuAspSerGlySerThrLysGluPhe                               670675680                                                                      TTCAGAAGATCCAAAATTGCTGTGTACGAGAAAATGTGGTCTTACATG2172                           PheArgArgSerLysIleAlaValTyrGluLysMetTrpSerTyrMet                               685690695700                                                                   AAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGACGGAGTG2220                           LysSerAlaGluProSerValPheThrLysThrThrAlaAspGlyVal                               705710715                                                                      GCCCGAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCA2268                           AlaArgValArgLysSerLysGlyLysPheAlaPheLeuLeuGluSer                               720725730                                                                      ACCATGAATGAGTACATTGAGCAGAGAAAACCATGTGATACGATGAAA2316                           ThrMetAsnGluTyrIleGluGlnArgLysProCysAspThrMetLys                               735740745                                                                      GTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAA2364                           ValGlyGlyAsnLeuAspSerLysGlyTyrGlyValAlaThrProLys                               750755760                                                                      GGCTCAGCATTAGGAAATGCTGTTAACCTGGCAGTATTAAAACTGAAT2412                           GlySerAlaLeuGlyAsnAlaValAsnLeuAlaValLeuLysLeuAsn                               765770775780                                                                   GAGCAAGGCCTCTTGGACAAATTGAAAAACAAATGGTGGTACGACAAA2460                           GluGlnGlyLeuLeuAspLysLeuLysAsnLysTrpTrpTyrAspLys                               785790795                                                                      GGAGAGTGCGGCAGCGGGGGCGGTGACTCCAAGGACAAGACCAGCGCT2508                           GlyGluCysGlySerGlyGlyGlyAspSerLysAspLysThrSerAla                               800805810                                                                      CTGAGCCTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGT2556                           LeuSerLeuSerAsnValAlaGlyValPheTyrIleLeuValGlyGly                               815820825                                                                      CTGGGGCTGGCCATGATGGTGGCTTTGATAGAATTCTGTTACAAATCA2604                           LeuGlyLeuAlaMetMetValAlaLeuIleGluPheCysTyrLysSer                               830835840                                                                      CGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTT2652                           ArgAlaGluSerLysArgMetLysLeuThrLysAsnThrGlnAsnPhe                               845850855860                                                                   AAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATACAGAGAA2700                           LysProAlaProAlaThrAsnThrGlnAsnTyrAlaThrTyrArgGlu                               865870875                                                                      GGCTACAACGTGTATGGAACAGAGAGTGTTAAGATCTAGGGATCCC2746                             GlyTyrAsnValTyrGlyThrGluSerValLysIle                                           880885                                                                         TTCCCACTGGAGGCATGTGATGAGAGGAAATCACCGAAAACGTGGCTGCTTCAAGGATCC2806               TGAGCCAGATTTCACTCTCCTTGGTGTCGGGCATGACACGAATATTGCTGATGGTGCAAT2866               GACCTTTCAATAGGAAAAACTGGTTTTTTTTTCCTTCAGTGCCTTATGGAACACTCTGAG2926               ACTCGCGACAATGCAAACCATCATTGAAATCTTTTTGCTTTGCTTGAAAAAAAAAAAAAA2986               AAA2989                                                                        (2) INFORMATION FOR SEQ ID NO:12:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 888 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:                                      MetGlyGlnSerValLeuArgAlaValPhePheLeuValLeuGlyLeu                               151015                                                                         LeuGlyHisSerHisGlyGlyPheProAsnThrIleSerIleGlyGly                               202530                                                                         LeuPheMetArgAsnThrValGlnGluHisSerAlaPheArgPheAla                               354045                                                                         ValGlnLeuTyrAsnThrAsnGlnAsnThrThrGluLysProPheHis                               505560                                                                         LeuAsnTyrHisValAspHisLeuAspSerSerAsnSerPheSerVal                               65707580                                                                       ThrAsnAlaPheCysSerGlnPheSerArgGlyValTyrAlaIlePhe                               859095                                                                         GlyPheTyrAspGlnMetSerMetAsnThrLeuThrSerPheCysGly                               100105110                                                                      AlaLeuHisThrSerPheValThrProSerPheProThrAspAlaAsp                               115120125                                                                      ValGlnPheValIleGlnMetArgProAlaLeuLysGlyAlaIleLeu                               130135140                                                                      SerLeuLeuGlyHisTyrLysTrpGluLysPheValTyrLeuTyrAsp                               145150155160                                                                   ThrGluArgGlyPheSerIleLeuGlnAlaIleMetGluAlaAlaVal                               165170175                                                                      GlnAsnAsnTrpGlnValThrAlaArgSerValGlyAsnIleLysAsp                               180185190                                                                      ValGlnGluPheArgArgIleIleGluGluMetAspArgArgGlnGlu                               195200205                                                                      LysArgTyrLeuIleAspCysGluValGluArgIleAsnThrIleLeu                               210215220                                                                      GluGlnValValIleLeuGlyLysHisSerArgGlyTyrHisTyrMet                               225230235240                                                                   LeuAlaAsnLeuGlyPheThrAspIleLeuLeuGluArgValMetHis                               245250255                                                                      GlyGlyAlaAsnIleThrGlyPheGlnIleValAsnAsnGluAsnPro                               260265270                                                                      MetValGlnGlnPheIleGlnArgTrpValArgLeuAspGluArgGlu                               275280285                                                                      PheProGluAlaLysAsnAlaProLeuLysTyrThrSerAlaLeuThr                               290295300                                                                      HisAspAlaIleLeuValIleAlaGluAlaPheArgTyrLeuArgArg                               305310315320                                                                   GlnArgValAspValSerArgArgGlySerAlaGlyAspCysLeuAla                               325330335                                                                      AsnProAlaValProTrpSerGlnGlyIleAspIleGluArgAlaLeu                               340345350                                                                      LysMetValGlnValGlnGlyMetThrGlyAsnIleGlnPheAspThr                               355360365                                                                      TyrGlyArgArgThrAsnTyrThrIleAspValTyrGluMetLysVal                               370375380                                                                      SerGlySerArgLysAlaGlyTyrTrpAsnGluTyrGluArgPheVal                               385390395400                                                                   ProPheSerAspGlnGlnIleSerAsnAspSerAlaSerSerGluAsn                               405410415                                                                      ArgThrIleValValThrThrIleLeuGluSerProTyrValMetTyr                               420425430                                                                      LysLysAsnHisGluGlnLeuGluGlyAsnGluArgTyrGluGlyTyr                               435440445                                                                      CysValAspLeuAlaTyrGluIleAlaLysHisValArgIleLysTyr                               450455460                                                                      LysLeuSerIleValGlyAspGlyLysTyrGlyAlaArgAspProGlu                               465470475480                                                                   ThrLysIleTrpAsnGlyMetValGlyGluLeuValTyrGlyArgAla                               485490495                                                                      AspIleAlaValAlaProLeuThrIleThrLeuValArgGluGluVal                               500505510                                                                      IleAspPheSerLysProPheMetSerLeuGlyIleSerIleMetIle                               515520525                                                                      LysLysProGlnLysSerLysProGlyValPheSerPheLeuAspPro                               530535540                                                                      LeuAlaTyrGluIleTrpMetCysIleValPheAlaTyrIleGlyVal                               545550555560                                                                   SerValValLeuPheLeuValSerArgPheSerProTyrGluTrpHis                               565570575                                                                      LeuGluAspAsnAsnGluGluProArgAspProGlnSerProProAsp                               580585590                                                                      ProProAsnGluPheGlyIlePheAsnSerLeuTrpPheSerLeuGly                               595600605                                                                      AlaPheMetGlnGlnGlyCysAspIleSerProArgSerLeuSerGly                               610615620                                                                      ArgIleValGlyGlyValTrpTrpPhePheThrLeuIleIleIleSer                               625630635640                                                                   SerTyrThrAlaAsnLeuAlaAlaPheLeuThrValGluArgMetVal                               645650655                                                                      SerProIleGluSerAlaGluAspLeuAlaLysGlnThrGluIleAla                               660665670                                                                      TyrGlyThrLeuAspSerGlySerThrLysGluPhePheArgArgSer                               675680685                                                                      LysIleAlaValTyrGluLysMetTrpSerTyrMetLysSerAlaGlu                               690695700                                                                      ProSerValPheThrLysThrThrAlaAspGlyValAlaArgValArg                               705710715720                                                                   LysSerLysGlyLysPheAlaPheLeuLeuGluSerThrMetAsnGlu                               725730735                                                                      TyrIleGluGlnArgLysProCysAspThrMetLysValGlyGlyAsn                               740745750                                                                      LeuAspSerLysGlyTyrGlyValAlaThrProLysGlySerAlaLeu                               755760765                                                                      GlyAsnAlaValAsnLeuAlaValLeuLysLeuAsnGluGlnGlyLeu                               770775780                                                                      LeuAspLysLeuLysAsnLysTrpTrpTyrAspLysGlyGluCysGly                               785790795800                                                                   SerGlyGlyGlyAspSerLysAspLysThrSerAlaLeuSerLeuSer                               805810815                                                                      AsnValAlaGlyValPheTyrIleLeuValGlyGlyLeuGlyLeuAla                               820825830                                                                      MetMetValAlaLeuIleGluPheCysTyrLysSerArgAlaGluSer                               835840845                                                                      LysArgMetLysLeuThrLysAsnThrGlnAsnPheLysProAlaPro                               850855860                                                                      AlaThrAsnThrGlnAsnTyrAlaThrTyrArgGluGlyTyrAsnVal                               865870875880                                                                   TyrGlyThrGluSerValLysIle                                                       885                                                                            (2) INFORMATION FOR SEQ ID NO: 13:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 1191 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: adult                                                 (C) TISSUE TYPE: brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 317..1191                                                        (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:                                      CGGGTCCTGACAGCCCCTTGGCCTCCCAGCATGGGGAAGCGTGAGGAGTTGCCCAGCAGT60                 GAGCAGCCCCCCTCACTCCTGGCCCCATGAGCCGCAGCCACAGGCAGCAGAGGAGGGCTA120                AGGAGAACTAGTCATAATCTTAAACCACCGAAACCTCTTTCCTTTTTTTTCTTTCTTTTC180                TTTCTTTTCTTTTTTTTTTTTTTTTTTTGGTTGATTTTAATTTTAGCGCCATCGTCTTCA240                ATGCTTCTCTGAACAGCCTTTAGGAAGAGTGCGAGAGAAAGAGAGAGAGCGCGCGCCAGG300                GAGAGGAGAAAAGAAGATGAGGATTATTTCCAGACAGATTGTCTTGTTA349                           MetArgIleIleSerArgGlnIleValLeuLeu                                              1510                                                                           TTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTCCGAGCAGCGTG397                            PheSerGlyPheTrpGlyLeuAlaMetGlyAlaPheProSerSerVal                               152025                                                                         CAAATAGGTGGTCTCTTCATCCGAAACACAGATCAGGAATACACTGCT445                            GlnIleGlyGlyLeuPheIleArgAsnThrAspGlnGluTyrThrAla                               303540                                                                         TTTCGATTAGCAATTTTTCTTCATAACACCAGCCCCAATGCGTCGGAA493                            PheArgLeuAlaIlePheLeuHisAsnThrSerProAsnAlaSerGlu                               455055                                                                         GCTCCTTTTAATTTGGTACCTCATGTGGACAACATTGAGACAGCCAAC541                            AlaProPheAsnLeuValProHisValAspAsnIleGluThrAlaAsn                               60657075                                                                       AGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTA589                            SerPheAlaValThrAsnAlaPheCysSerGlnTyrSerArgGlyVal                               808590                                                                         TTTGCCATTTTTGGACTCTATGATAAGAGGTCGGTACATACCTTGACC637                            PheAlaIlePheGlyLeuTyrAspLysArgSerValHisThrLeuThr                               95100105                                                                       TCATTCTGCAGCGCCTTACATATCTCCCTCATCACACCAAGTTTCCCT685                            SerPheCysSerAlaLeuHisIleSerLeuIleThrProSerPhePro                               110115120                                                                      ACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAGACCTTCGTTACGA733                            ThrGluGlyGluSerGlnPheValLeuGlnLeuArgProSerLeuArg                               125130135                                                                      GGAGCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGTC781                            GlyAlaLeuLeuSerLeuLeuAspHisTyrGluTrpAsnCysPheVal                               140145150155                                                                   TTCCTGTATGACACAGACAGGGGATACTCGATACTCCAAGCTATTTTG829                            PheLeuTyrAspThrAspArgGlyTyrSerIleLeuGlnAlaIleLeu                               160165170                                                                      GAAAAAGCAGGACAAAATGGTTGGCATGTCAGCGCTATATGTGTGGAA877                            GluLysAlaGlyGlnAsnGlyTrpHisValSerAlaIleCysValGlu                               175180185                                                                      AATTTTAATGATGTCAGCTATAGGCAACTTCTAGAAGAACTTGACAGA925                            AsnPheAsnAspValSerTyrArgGlnLeuLeuGluGluLeuAspArg                               190195200                                                                      AGACAAGAGAAGAAGTTTGTAATAGACTGTGAGATAGAGAGACTTCAA973                            ArgGlnGluLysLysPheValIleAspCysGluIleGluArgLeuGln                               205210215                                                                      AACATATTAGAACAGATTGTAAGTGTTGGAAAGCATGTTAAAGGCTAC1021                           AsnIleLeuGluGlnIleValSerValGlyLysHisValLysGlyTyr                               220225230235                                                                   CATTATATCATTGCAAACTTGGGATTCAAGGATATTTCTCTTGAGAGG1069                           HisTyrIleIleAlaAsnLeuGlyPheLysAspIleSerLeuGluArg                               240245250                                                                      TTTATACATGGTGGAGCCAATGTTACTGGATTCCAGTTGGTGGATTTT1117                           PheIleHisGlyGlyAlaAsnValThrGlyPheGlnLeuValAspPhe                               255260265                                                                      AATACACCTATGGTAATCAAACTAATGGATCGCTGGAAGAAACTAGAT1165                           AsnThrProMetValIleLysLeuMetAspArgTrpLysLysLeuAsp                               270275280                                                                      CAGAGAGAGTATCCAGGATCTGAGCC1191                                                 GlnArgGluTyrProGlySerGlu                                                       285290                                                                         (2) INFORMATION FOR SEQ ID NO:14:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 291 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:                                      MetArgIleIleSerArgGlnIleValLeuLeuPheSerGlyPheTrp                               151015                                                                         GlyLeuAlaMetGlyAlaPheProSerSerValGlnIleGlyGlyLeu                               202530                                                                         PheIleArgAsnThrAspGlnGluTyrThrAlaPheArgLeuAlaIle                               354045                                                                         PheLeuHisAsnThrSerProAsnAlaSerGluAlaProPheAsnLeu                               505560                                                                         ValProHisValAspAsnIleGluThrAlaAsnSerPheAlaValThr                               65707580                                                                       AsnAlaPheCysSerGlnTyrSerArgGlyValPheAlaIlePheGly                               859095                                                                         LeuTyrAspLysArgSerValHisThrLeuThrSerPheCysSerAla                               100105110                                                                      LeuHisIleSerLeuIleThrProSerPheProThrGluGlyGluSer                               115120125                                                                      GlnPheValLeuGlnLeuArgProSerLeuArgGlyAlaLeuLeuSer                               130135140                                                                      LeuLeuAspHisTyrGluTrpAsnCysPheValPheLeuTyrAspThr                               145150155160                                                                   AspArgGlyTyrSerIleLeuGlnAlaIleLeuGluLysAlaGlyGln                               165170175                                                                      AsnGlyTrpHisValSerAlaIleCysValGluAsnPheAsnAspVal                               180185190                                                                      SerTyrArgGlnLeuLeuGluGluLeuAspArgArgGlnGluLysLys                               195200205                                                                      PheValIleAspCysGluIleGluArgLeuGlnAsnIleLeuGluGln                               210215220                                                                      IleValSerValGlyLysHisValLysGlyTyrHisTyrIleIleAla                               225230235240                                                                   AsnLeuGlyPheLysAspIleSerLeuGluArgPheIleHisGlyGly                               245250255                                                                      AlaAsnValThrGlyPheGlnLeuValAspPheAsnThrProMetVal                               260265270                                                                      IleLysLeuMetAspArgTrpLysLysLeuAspGlnArgGluTyrPro                               275280285                                                                      GlySerGlu                                                                      290                                                                            (2) INFORMATION FOR SEQ ID NO: 15:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 1191 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA to mRNA                                               (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Homo sapiens                                                     (B) DEVELOPMENTAL STAGE: adult                                                 (C) TISSUE TYPE: brain                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 317..1191                                                        (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:                                      CGGGTCCTGACAGCCCCTTGGCCTCCCAGCATGGGGAAGCGTGAGGAGTTGCCCAGCAGT60                 GAGCAGCCCCCCTCACTCCTGGCCCCATGAGCCGCAGCCACAGGCAGCAGAGGAGGGCTA120                AGGAGAACTAGTCATAATCTTAAACCACCGAAACCTCTTTCCTTTTTTTTCTTTCTTTTC180                TTTCTTTTCTTTTTTTTTTTTTTTTTTTGGTTGATTTTAATTTTAGCGCCATCGTCTTCA240                ATGCTTCTCTGAACAGCCTTTAGGAAGAGTGCGAGAGAAAGAGAGAGAGCGCGCGCCAGG300                GAGAGGAGAAAAGAAGATGAGGATTATTTCCAGACAGATTGTCTTGTTA349                           MetArgIleIleSerArgGlnIleValLeuLeu                                              1510                                                                           TTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTCCGAGCAGCGTG397                            PheSerGlyPheTrpGlyLeuAlaMetGlyAlaPheProSerSerVal                               152025                                                                         CAAATAGGTGGTCTCTTCATCCGAAACACAGATCAGGAATACACTGCT445                            GlnIleGlyGlyLeuPheIleArgAsnThrAspGlnGluTyrThrAla                               303540                                                                         TTTCGATTAGCAATTTTTCTTCATAACACCAGCCCCAATGCGTCGGAA493                            PheArgLeuAlaIlePheLeuHisAsnThrSerProAsnAlaSerGlu                               455055                                                                         GCTCCTTTTAATTTGGTACCTCATGTGGACAACATTGAGACAGCCAAC541                            AlaProPheAsnLeuValProHisValAspAsnIleGluThrAlaAsn                               60657075                                                                       AGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTA589                            SerPheAlaValThrAsnAlaPheCysSerGlnTyrSerArgGlyVal                               808590                                                                         TTTGCCATTTTTGGACTCTATGATAAGAGGTCGGTACATACCTTGACC637                            PheAlaIlePheGlyLeuTyrAspLysArgSerValHisThrLeuThr                               95100105                                                                       TCATTCTGCAGCGCCTTACATATCTCCCTCATCACACCAAGTTTCCCT685                            SerPheCysSerAlaLeuHisIleSerLeuIleThrProSerPhePro                               110115120                                                                      ACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAGACCTTCGTTACGA733                            ThrGluGlyGluSerGlnPheValLeuGlnLeuArgProSerLeuArg                               125130135                                                                      GGAGCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGTC781                            GlyAlaLeuLeuSerLeuLeuAspHisTyrGluTrpAsnCysPheVal                               140145150155                                                                   TTCCTGTATGACACAGACAGGGGATACTCGATACTCCAAGCTATTTTG829                            PheLeuTyrAspThrAspArgGlyTyrSerIleLeuGlnAlaIleLeu                               160165170                                                                      GAAAAAGCAGGACAAAATGGTTGGCATGTCAGCGCTATATGTGTGGAA877                            GluLysAlaGlyGlnAsnGlyTrpHisValSerAlaIleCysValGlu                               175180185                                                                      AATTTTAATGATGTCAGCTATAGGCAACTTCTAGAAGAACTTGACAGA925                            AsnPheAsnAspValSerTyrArgGlnLeuLeuGluGluLeuAspArg                               190195200                                                                      AGACAAGAGAAGAAGTTTGTAATAGACTGTGAGATAGAGAGACTTCAA973                            ArgGlnGluLysLysPheValIleAspCysGluIleGluArgLeuGln                               205210215                                                                      AACATATTAGAACAGATTGTAAGTGTTGGAAAGCATGTTAAAGGCTAC1021                           AsnIleLeuGluGlnIleValSerValGlyLysHisValLysGlyTyr                               220225230235                                                                   CATTATATCATTGCAAACTTGGGATTCAAGGATATTTCTCTTGAGAGG1069                           HisTyrIleIleAlaAsnLeuGlyPheLysAspIleSerLeuGluArg                               240245250                                                                      TTTATACATGGTGGAGCCAATGTTACTGGATTCCAGTTGGTGGATTTT1117                           PheIleHisGlyGlyAlaAsnValThrGlyPheGlnLeuValAspPhe                               255260265                                                                      AATACACCTATGGTAATCAAACTAATGGATCGCTGGAAGAAACTAGAT1165                           AsnThrProMetValIleLysLeuMetAspArgTrpLysLysLeuAsp                               270275280                                                                      CAGAGAGAGTATCCAGGATCTGAGCC1191                                                 GlnArgGluTyrProGlySerGlu                                                       285290                                                                         (2) INFORMATION FOR SEQ ID NO: 16:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 291 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:                                      MetArgIleIleSerArgGlnIleValLeuLeuPheSerGlyPheTrp                               151015                                                                         GlyLeuAlaMetGlyAlaPheProSerSerValGlnIleGlyGlyLeu                               202530                                                                         PheIleArgAsnThrAspGlnGluTyrThrAlaPheArgLeuAlaIle                               354045                                                                         PheLeuHisAsnThrSerProAsnAlaSerGluAlaProPheAsnLeu                               505560                                                                         ValProHisValAspAsnIleGluThrAlaAsnSerPheAlaValThr                               65707580                                                                       AsnAlaPheCysSerGlnTyrSerArgGlyValPheAlaIlePheGly                               859095                                                                         LeuTyrAspLysArgSerValHisThrLeuThrSerPheCysSerAla                               100105110                                                                      LeuHisIleSerLeuIleThrProSerPheProThrGluGlyGluSer                               115120125                                                                      GlnPheValLeuGlnLeuArgProSerLeuArgGlyAlaLeuLeuSer                               130135140                                                                      LeuLeuAspHisTyrGluTrpAsnCysPheValPheLeuTyrAspThr                               145150155160                                                                   AspArgGlyTyrSerIleLeuGlnAlaIleLeuGluLysAlaGlyGln                               165170175                                                                      AsnGlyTrpHisValSerAlaIleCysValGluAsnPheAsnAspVal                               180185190                                                                      SerTyrArgGlnLeuLeuGluGluLeuAspArgArgGlnGluLysLys                               195200205                                                                      PheValIleAspCysGluIleGluArgLeuGlnAsnIleLeuGluGln                               210215220                                                                      IleValSerValGlyLysHisValLysGlyTyrHisTyrIleIleAla                               225230235240                                                                   AsnLeuGlyPheLysAspIleSerLeuGluArgPheIleHisGlyGly                               245250255                                                                      AlaAsnValThrGlyPheGlnLeuValAspPheAsnThrProMetVal                               260265270                                                                      IleLysLeuMetAspArgTrpLysLysLeuAspGlnArgGluTyrPro                               275280285                                                                      GlySerGlu                                                                      290                                                                            (2) INFORMATION FOR SEQ ID NO: 17:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 20 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17:                                      ATCTATGATTGGACCTGGGC20                                                         (2) INFORMATION FOR SEQ ID NO: 18:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 24 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18:                                      ACATCTGCTCTTCCATAGACCAGC24                                                     (2) INFORMATION FOR SEQ ID NO: 19:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 39 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19:                                      TGCGATAAGCTTATGCAGCACATTTTTGCCTTCTTCTGC39                                      (2) INFORMATION FOR SEQ ID NO: 20:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 24 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20:                                      ATGCCATTCCAGGCCTTCGTGTCA24                                                     (2) INFORMATION FOR SEQ ID NO: 21:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21:                                      GATGGAAAATACGGAGCCCGA21                                                        (2) INFORMATION FOR SEQ ID NO: 22:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 21 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22:                                      GCTGGGGAGCCGAGCCTGCTC21                                                        (2) INFORMATION FOR SEQ ID NO: 23:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 24 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23:                                      TGACACGAAGGCCTGGAATGGCAT24                                                     (2) INFORMATION FOR SEQ ID NO: 24:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 39 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24:                                      TGCGATGAATTCTTACAATCCCGTGGCTCCCAAGGGCAT39                                      (2) INFORMATION FOR SEQ ID NO: 25:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 22 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:                                      TACTTGGGTCTCTTCCAGTCCA22                                                       (2) INFORMATION FOR SEQ ID NO: 26:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 24 base pairs                                                      (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: genomic DNA                                                (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26:                                      TGTGTGGTCTCGAGCATCACTATT24                                                     __________________________________________________________________________ 

We claim:
 1. An isolated polynucleotide coding for a variant of a glutamate receptor subunit, the sequence of said polynucleotide being selected from the group consisting of:a) a polynucleotide comprising the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 3; b) a polynucleotide sequence coding for a protein comprising the amino acid sequence shown in SEQ ID NO: 2 or SEQ ID NO: 4; and c) a polynucleotide comprising a nucleotide sequence fully complementary to the nucleotide sequence of a) or b).
 2. A method of preparing a glutamate receptor subunit, comprising the steps ofconstructing a recombinant vector comprising the DNA sequence defined in claim 1 which codes for a glutamate receptor subunit; transforming a compatible host with the recombinant vector such that the DNA sequence coding for the glutamate receptor subunit can be expressed by the host; culturing the transformed host in a suitable growth medium to produce the glutamate receptor subunit; and recovering the glutamate receptor subunit from the medium.
 3. The isolated polynucleotide of claim 1 wherein the polynucleotide comprises DNA.
 4. The isolated polynucleotide of claim 1 wherein the polynucleotide comprises RNA.
 5. A method for identifying functional ligands for glutamate receptors, which comprises tranfecting cells with one or more DNA sequences coding for a glutamate receptor as claimed in claim 1, and detecting the effect on the signal transduction pathway caused in these cells by binding of the ligands to the receptor by a reporter system. 